Our current knowledge of the clinical burden, biology, and transmission of Plasmodium malariae is extremely scarce. To start addressing some of those questions, we experimentally infected Anopheles gambiae mosquitoes with fresh P. malariae isolates obtained from asymptomatic individuals in Lambaréné, Gabon. The proportion of mosquitoes infected via direct membrane feeding assay with either P. malariae mono-infections (16%; 19/121) or co-infections (28%; 31/112) was higher after serum replacement compared to parallel groups without serum replacement (4%, 4/102; and 4%, 2/45; p<0.01 respectively). Our results show that isolates from asymptomatic carriers can be used for experimental studies of P. malariae transmission.

中文翻译：

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疟原虫向冈比亚按蚊的实验传播。

我们目前对疟原虫的临床负担、生物学和传播的了解极为匮乏。为了开始解决其中的一些问题，我们用从加蓬兰巴雷内的无症状个体中获得的新鲜疟原虫分离株实验性地感染了冈比亚按蚊。与未更换血清的平行组相比，更换血清后通过直接膜喂养试验感染疟原虫单一感染 (16%; 19/121) 或合并感染 (28%; 31/112)的蚊子比例更高（4%，4/102；和 4%，2/45；分别为 p<0.01）。我们的结果表明，无症状携带者的分离物可用于疟原虫的实验研究 传播。