A number of soybean varieties traditionally bred for resistance to various soybean arthropod pests have been identified as resistant to Megacopta cribraria (F.) (Hemiptera: Plataspidae). However, the mechanisms of host-plant resistance (HPR) in this system are not understood. The goal of this study was to identify the mechanisms of resistance by examining the role of plant volatile organic compounds (VOCs) and free amino acids (FAAs) among 16 soybean varieties. Choice and no-choice cage experiments identified several soybean varieties that demonstrated antixenosis as well as antibiosis. However, resistance varied over time in certain soybean varieties, such as N02-7002 and PI567352B. Mean nymph number from choice experiments had positive correlations with the FAAs asparagine, tryptophan, alanine, phenylanaline, and serine; negative correlation with leucine and threonine. Four plant volatiles, hexanal, 2-pentylfuran, beta-cyclocitral, and cis-9-hexadecenal, were positively correlated with subsequent nymph development, whereas n-hexadecenoic acid was negatively correlated with nymph number only, in adult choice cage experiments. This study contributes to understanding the mechanisms of HPR through associations with plant VOCs and FAAs in relation to M. cribraria development and provides useful knowledge for developing soybean varieties for M. cribraria management.

中文翻译：

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大豆寄主植物对 Megacopta cribraria（半翅目：Plataspidae）的抗性机制

许多传统上培育的抗各种大豆节肢动物害虫的大豆品种已被鉴定为对 Megacopta cribraria (F.)（半翅目： Plataspidae）具有抗性。然而，该系统中宿主植物抗性 (HPR) 的机制尚不清楚。本研究的目的是通过检查植物挥发性有机化合物 (VOC) 和游离氨基酸 (FAA) 在 16 个大豆品种中的作用来确定抗性机制。选择和非选择笼实验确定了几种表现出抗排毒和抗菌作用的大豆品种。然而，某些大豆品种的抗性随时间而变化，例如 N02-7002 和 PI567352B。选择实验的平均若虫数量与 FAA 天冬酰胺、色氨酸、丙氨酸、苯丙氨酸和丝氨酸呈正相关；与亮氨酸和苏氨酸呈负相关。在成人选择笼实验中，四种植物挥发物己醛、2-戊基呋喃、β-环柠檬醛和顺式 9-十六烯醛与随后的若虫发育呈正相关，而正十六烯酸仅与若虫数量呈负相关。本研究通过与植物 VOC 和 FAA 的关联与 M. cribraria 发育相关，有助于了解 HPR 的机制，并为开发用于 M. cribraria 管理的大豆品种提供有用的知识。