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Shotgun proteomics analysis of SARS-CoV-2-infected cells and how it can optimize whole viral particle antigen production for vaccines.
Emerging Microbes & Infections ( IF 13.2 ) Pub Date : 2020-07-27 , DOI: 10.1080/22221751.2020.1791737
Lucia Grenga 1 , Fabrice Gallais 1 , Olivier Pible 1 , Jean-Charles Gaillard 1 , Duarte Gouveia 1 , Hélène Batina 1 , Niza Bazaline 1 , Sylvie Ruat 1 , Karen Culotta 1 , Guylaine Miotello 1 , Stéphanie Debroas 1 , Marie-Anne Roncato 1 , Gérard Steinmetz 1 , Charlotte Foissard 1 , Anne Desplan 1 , Béatrice Alpha-Bazin 1 , Christine Almunia 1 , Fabienne Gas 1 , Laurent Bellanger 1 , Jean Armengaud 1
Affiliation  

ABSTRACT

Severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) has resulted in a pandemic and is continuing to spread rapidly around the globe. No effective vaccine is currently available to prevent COVID-19, and intense efforts are being invested worldwide into vaccine development. In this context, all technology platforms must overcome several challenges resulting from the use of an incompletely characterized new virus. These include finding the right conditions for virus amplification for the development of vaccines based on inactivated or attenuated whole viral particles. Here, we describe a shotgun tandem mass spectrometry workflow, the data produced can be used to guide optimization of the conditions for viral amplification. In parallel, we analysed the changes occurring in the host cell proteome following SARS-CoV-2 infection to glean information on the biological processes modulated by the virus that could be further explored as potential drug targets to deal with the pandemic.



中文翻译:

gun弹枪蛋白质组学分析受SARS-CoV-2感染的细胞,以及它如何优化疫苗的整个病毒颗粒抗原生产。

摘要

严重的与呼吸道综合症相关的冠状病毒2(SARS-CoV-2)导致大流行,并继续在全球迅速传播。目前尚无有效的疫苗可预防COVID-19,并且全球在疫苗开发方面投入了巨大的努力。在这种情况下,所有技术平台都必须克服因使用特征不完全的新病毒而带来的若干挑战。其中包括根据灭活或减毒的完整病毒颗粒,找到合适的病毒扩增条件以开发疫苗。在这里,我们描述了a弹枪串联质谱工作流程,所产生的数据可用于指导病毒扩增条件的优化。在平行下,

更新日期:2020-07-27
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