This work presents the development and validation of a simple, rapid, and cost-effective spectrophotometric method for quantitative analysis of uric acid in biological samples. The method relies upon uric acid-led reduction of Fe(III) to Fe(II) of sample/standard solutions which stoichiometrically engages ferrozine to form a magenta-colored complex. Different parameters including pH, metal and chelator concentrations, temperature, etc., were optimized for the maximum intensity and stability of the complex. The uric acid concentrations of synthetic/plasma solutions were determined by comparing the color intensity of Fe(ferrozine)₃ ²⁺ complex produced by test solution with the standard curve formed by known uric acid concentrations. The method was validated in accordance with ICH guidelines and subjected to human plasma analysis. The results obtained were compared with a reference (enzymatic) method which revealed that there was no significant difference between the two methods at 95% confidence level. The method is highly specific, precise, linear, accurate, and robust.

这项工作提出了一种简单，快速且具有成本效益的分光光度法对生物样品中尿酸进行定量分析的方法开发和验证。该方法依赖于尿酸引导下的样品/标准溶液中的Fe（III）还原为Fe（II），该溶液以化学计量方式与二氢呋喃结合形成品红色络合物。针对复合物的最大强度和稳定性，对包括pH，金属和螯合剂浓度，温度等在内的不同参数进行了优化。通过比较Fe（ferrozine）₃ ²⁺的颜色强度来确定合成/血浆溶液中的尿酸浓度由测试溶液产生的配合物，其标准曲线由已知的尿酸浓度形成。该方法已按照ICH指南进行了验证，并进行了人体血浆分析。将获得的结果与参考（酶促）方法进行比较，该方法表明在95％置信水平下两种方法之间没有显着差异。该方法具有高度的特异性，精确性，线性，准确性和鲁棒性。