Radiation therapy is part of the standard of care for gliomas and kills a subset of tumor cells, while also altering the tumor microenvironment. Tumor cells with stem‐like properties preferentially survive radiation and give rise to glioma recurrence. Various techniques for enriching and quantifying cells with stem‐like properties have been used, including the fluorescence activated cell sorting (FACS)‐based side population (SP) assay, which is a functional assay that enriches for stem‐like tumor cells. In these analyses, mouse models of glioma have been used to understand the biology of this disease and therapeutic responses, including the radiation response. We present combined SP analysis and single‐cell RNA sequencing of genetically‐engineered mouse models of glioma to show a time course of cellular response to radiation. We identify and characterize two distinct tumor cell populations that are inherently radioresistant and also distinct effects of radiation on immune cell populations within the tumor microenvironment.

中文翻译：

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多模式单细胞分析揭示了小鼠神经胶质瘤中不同的抗辐射干细胞样和祖细胞群。

放射治疗是胶质瘤护理标准的一部分，可杀死一部分肿瘤细胞，同时也会改变肿瘤微环境。具有干细胞样特性的肿瘤细胞优先在辐射中存活并导致神经胶质瘤复发。已经使用了多种技术来富集和量化具有干细胞样特性的细胞，包括基于荧光激活细胞分选 (FACS) 的侧群 (SP) 分析，这是一种富集干细胞样肿瘤细胞的功能分析。在这些分析中，胶质瘤的小鼠模型已被用于了解这种疾病的生物学和治疗反应，包括辐射反应。我们展示了神经胶质瘤基因工程小鼠模型的 SP 分析和单细胞 RNA 测序的组合，以显示细胞对辐射反应的时间过程。