The organophosphorus pesticides (OPPs) commonly used in agricultural practices can pose a risk of potential exposure to humans via food consumption. We describe an analytical method for solid‐phase extraction coupled with high‐performance liquid chromatography–diode array detector (SPE–HPLC–DAD) for the detection of OPPs (quinalphos, diazinon, and chlorpyrifos) in rice grains. The isolation of targeted residues was initiated with double extraction before SPE–HPLC–DAD, crucially reducing matrix interferences and detecting a wide range of multiple residues in rice grains. Coefficients of 0.9968 to 0.9991 showed a strong linearity, with limits of detection and quantification ranging from 0.36 to 0.68 µg/kg and from 1.20 to 2.28 µg/kg, respectively. High recoveries (80.4–110.3%) were observed at 3 spiking levels (50, 100, and 200 µg/kg), indicating good accuracy. The relative standard deviations of all residues (0.19–8.66%) validated the method precision. Sample analysis of 10 rice grain types (n = 30) available in the Asian market revealed that quinalphos, diazinon, and chlorpyrifos at concentrations of 1.08, 1.11, and 1.79 µg/kg, respectively, remained far below the maximum residue limits (0.01–0.5 mg/kg). However, regular monitoring is necessary to confirm that multiresidue occurrence remains below permissible limits while controlling pests. Environ Toxicol Chem 2020;39:1908–1917. © 2020 SETAC

中文翻译：

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商业化亚洲稻米中的有机磷农药多残留。

农业实践中常用的有机磷农药（OPPs）可能会通过食用食物而对人类构成潜在的风险。我们描述了一种固相萃取与高效液相色谱-二极管阵列检测器（SPE-HPLC-DAD）结合使用的检测方法，用于检测稻米中的OPP（喹啉，二嗪农和毒死rif）。在SPE-HPLC-DAD之前，通过两次萃取开始了目标残留物的分离，从而极大地减少了基质干扰并检测了水稻籽粒中多种多样的残留物。系数0.9968至0.9991表现出很强的线性，检出限和定量限分别为0.36至0.68 µg / kg和1.20至2.28 µg / kg。在三种加标水平（50、100和200 µg / kg）下，观察到高回收率（80.4–110.3％），表明准确度高。所有残留物的相对标准偏差（0.19–8.66％）验证了方法的准确性。十种水稻籽粒的样品分析（n  = 30），在亚洲市场上可以看到，分别为1.08、1.11和1.79 µg / kg的喹啉磷，二嗪农和毒死rif仍远低于最大残留限量（0.01-0.5 mg / kg）。但是，必须定期进行监测，以确认在控制害虫时多残基的发生率保持在允许的限值以下。环境毒性化学2020； 39：1908–1917。©2020 SETAC