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Comparative analysis of biological effects of molybdenum(IV) sulfide in the form of nano- and microparticles on human hepatoma HepG2 cells grown in 2D and 3D models.
Toxicology in Vitro ( IF 3.2 ) Pub Date : 2020-07-05 , DOI: 10.1016/j.tiv.2020.104931
Z Sobańska 1 , K Domeradzka-Gajda 1 , M Szparaga 1 , J Grobelny 2 , E Tomaszewska 2 , K Ranoszek-Soliwoda 2 , G Celichowski 2 , L Zapór 3 , K Kowalczyk 1 , M Stępnik 1
Affiliation  

Significance of MoS2 nanoparticles as a lubricant or drug carriers indicates the need to assess their safety. In the study we analyzed the effects of MoS2 nano- and microparticles and their internalization in vitro, using 2D and 3D culture models of human hepatoma HepG2 cell line.

MoS2 micro- and nanoparticles were characterized with high resolution electron microscopy (HR-SEM), X-ray diffraction (XRD) and Energy Dispersive X-Ray Spectroscopy (EDS). The cells were exposed to a range of concentrations of the nano-and microparticles suspensions (maximum of 250 μg/mL) for 72 h. Cell viability was assessed using WST-1 reduction test and LDH release assay. Particle internalization was analyzed using scanning transmission electron microscopy (STEM).

The nanoparticles were internalized into the 2D and 3D cultured cells, in spheroids more efficiently into the outer layer. For microparticles mainly particles of less than 1 μm in diameter underwent internalization. This process, however, did not affect cell viability as measured with the WST-1 and LDH assays. STEM observation showed well preserved integrity of the cell membrane and no apparent cytotoxic effect. Although the particles seemed to be safely sequestered in vacuoles or the cytoplasm, their fate and eventual biological effects are not certain and deserve further studies.



中文翻译:

纳米和微粒形式的硫化钼(IV)对在2D和3D模型中生长的人肝癌HepG2细胞的生物学效应的比较分析。

MoS 2纳米颗粒作为润滑剂或药物载体的重要性表明需要评估其安全性。在这项研究中,我们使用人肝癌HepG2细胞系的2D和3D培养模型分析了MoS 2纳米和微粒的作用及其体外内在化。

MoS 2微米和纳米颗粒通过高分辨率电子显微镜(HR-SEM),X射线衍射(XRD)和能量色散X射线光谱(EDS)进行了表征。将细胞暴露于一定浓度的纳米和微粒悬浮液(最大250μg/ mL)中72小时。使用WST-1减少测试和LDH释放测定法评估细胞活力。使用扫描透射电子显微镜(STEM)分析颗粒的内在化。

纳米颗粒被内化到2D和3D培养的细胞中,更有效地以球状体进入外层。对于微粒,主要对直径小于1μm的颗粒进行内部化处理。但是,此过程并不影响细胞活力,如WST-1和LDH分析所测。STEM观察显示细胞膜完整保存完好,没有明显的细胞毒性作用。尽管这些颗粒似乎被安全地隔离在液泡或细胞质中,但它们的命运和最终的生物学作用尚不确定,值得进一步研究。

更新日期:2020-07-13
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