Research question

Long non-coding RNA (lncRNA) do not show protein translation but do have gene regulatory functions in several disease states. Studies have shown that lncRNA differ in overweight women with polycystic ovary syndrome (PCOS), increased insulin resistance and hyperandrogenaemia. The objective of this study was to determine the lncRNA in serum in age- and weight-matched non-obese women with and without PCOS.

Methods

In this prospective pilot cohort study, lncRNA were measured in serum in 13 non-obese women with PCOS and 10 control women undergoing IVF.

Results

There was no difference between groups in terms of age, body mass index or insulin resistance. Women with PCOS showed a higher free androgen index (FAI; P = 0.03) and anti-Müllerian hormone (AMH) concentration (P = 0.001). A total of 29 lncRNA (P ≤ 0.05) differed between PCOS groups. lncRNA AC095350.1 correlated with age (r = 0.79, P = 0.04), but no correlation was seen between the significantly different lncRNA and FAI or AMH values. Functional pathway assessment using the Ingenuity Pathway Assessment tool showed no relationships for the lncRNA.

Conclusion

lncRNA in serum differed between non-obese women with PCOS and the control group, and the pattern of expression differed from that reported in obese women with PCOS from the same ethnic population; however, it but did not correlate with androgen or insulin resistance.

中文翻译：

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患有多囊卵巢综合征和体重匹配对照的非肥胖女性的长链非编码 RNA 表达。

研究问题

长链非编码 RNA (lncRNA) 不显示蛋白质翻译，但在几种疾病状态下确实具有基因调控功能。研究表明，lncRNA 在患有多囊卵巢综合征 (PCOS)、胰岛素抵抗增加和高雄激素血症的超重女性中有所不同。本研究的目的是确定年龄和体重匹配的患有和不患有 PCOS 的非肥胖女性血清中的 lncRNA。

方法

在这项前瞻性先导队列研究中，在 13 名患有 PCOS 的非肥胖女性和 10 名接受 IVF 的对照女性的血清中测量了 lncRNA。

结果

各组在年龄、体重指数或胰岛素抵抗方面没有差异。患有 PCOS 的女性表现出更高的游离雄激素指数（FAI；P  = 0.03）和抗苗勒管激素（AMH）浓度（P  = 0.001）。 PCOS 组间共有 29 个 lncRNA ( P ≤ 0.05) 存在差异。lncRNA AC095350.1 与年龄相关（r  = 0.79，P  = 0.04），但在显着不同的 lncRNA 与 FAI 或 AMH 值之间未观察到相关性。使用 Ingenuity Pathway Assessment 工具进行的功能通路评估显示与 lncRNA 没有关系。

结论

非肥胖PCOS女性与对照组血清中lncRNA的表达存在差异，其表达模式与同族群肥胖PCOS女性报道的表达模式不同；然而，它与雄激素或胰岛素抵抗无关。