Abstract Lateral flow immunoassays (LFIAs) provide a powerful tool for rapid real-time assay of cancer biomarkers, which is vital for cancer detection and treatment follow-up. Lanthanide-based LFIAs is one of the most widely used methods, especially Eu (III) chelates, which possess distinctive and attractive characteristics, such as time-resolved fluorescence and large Stokes shift. Herein, we adopted a new one-step mini-emulsion polymerization method to synthesize carboxyl-modified fluorescent microsphere (OS-EuCM), which shows good stability, resistance to non-specific adhesion and uniform particle size distribution compared with traditional microspheres synthesized through the swelling method. Benefiting from the above advantages, OS-EuCM was successfully used in LFIAs to detect tumor marker Alpha-fetoprotein with high sensitivity and selectivity in concentration as high as 320 ng/mL, as well as a detection limit of 0.683 ng/mL. This lanthanide-based microsphere holds great potential for rapid point-of-care screening and clinical application.

中文翻译：

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用于灵敏侧流免疫测定的一步聚合镧系元素聚苯乙烯微球

摘要 横向流动免疫分析 (LFIA) 为快速实时分析癌症生物标志物提供了强大的工具，这对于癌症检测和治疗随访至关重要。基于镧系元素的 LFIA 是应用最广泛的方法之一，尤其是 Eu (III) 螯合物，它具有独特而有吸引力的特性，例如时间分辨荧光和大斯托克斯位移。在此，我们采用了一种新的一步微乳液聚合方法合成了羧基修饰的荧光微球（OS-EuCM），与通过以下方法合成的传统微球相比，该微球具有良好的稳定性、抗非特异性粘附性和均匀的粒径分布。溶胀法。得益于以上优势，OS-EuCM 已成功用于 LFIA 以高灵敏度和选择性检测肿瘤标志物甲胎蛋白，浓度高达 320 ng/mL，检测限为 0.683 ng/mL。这种基于镧系元素的微球在快速床旁筛查和临床应用方面具有巨大潜力。