The roots of are also a valuable resource in addition to its flowers and fruits. In this study, the HPLC-MS/MS method used for analyzing the chemical constituents in roots extract was developed, which showed high sensitivity for both qualitative and quantitative analyses. Thirty-two compounds were first discovered in roots, one compound of which was reported for the first time. The simultaneous determination method for acteoside, isoacteoside, oleuropein and phillyrin was validated to be sensitive and accurate. Then it was applied to determine the content of bioactive components in roots from different cultivars. The content of oleuropein and phillyrin in the twelve batches was relatively stable, while the content of acteoside and isoacteoside varied greatly. Moreover, the therapeutic material basis and mechanism of roots exerting its traditional pharmacodynamics were analyzed by network pharmacology. The results showed that roots might be effective for the treatment of inflammation, cardiovascular diseases, cancer, and rheumatoid arthritis, which is consistent with the traditional pharmacodynamics of roots. This work can provide an analytical method for the comprehensive development of roots.

中文翻译：

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HPLC-MS/MS 法鉴定和定量桂花根中的生物活性成分

除了花和果实之外，根也是一种宝贵的资源。在本研究中，开发了用于分析根提取物中化学成分的 HPLC-MS/MS 方法，该方法对定性和定量分析均显示出高灵敏度。首次在根中发现32种化合物，其中1种化合物为首次报道。验证了同时测定毛叶松苷、异叶松叶苷、橄榄苦苷和连翘苷的方法灵敏、准确。然后应用该方法测定不同品种根中生物活性成分的含量。 12个批次中橄榄苦苷和连翘苷的含量相对稳定，而类升麻苷和异类升麻苷的含量变化较大。并通过网络药理学分析了根茎发挥其传统药效的药用物质基础和作用机制。结果表明，根可能有效治疗炎症、心血管疾病、癌症和类风湿关节炎，这与根的传统药效一致。本工作可为根系的全面发育提供分析方法。