In this study, the microcrystalline cellulose/metal-organic framework 199 hybrid (MCC/MOF-199) was applied as sorbent for the dispersive micro-solid phase-extraction (D-μSPE) of chlorophenols. The D-μSPE method combined with high-performance liquid chromatography- ultraviolet detection (HPLC-UV) was employed to determine of four chlorophenols including 2-chlorophenol (2-CP), 4-chlorophenol (4-CP), 2,3-dichlorophenol (2,3-DCP), and 2,5-dichlorophenol (2,5-DCP) in aqueous. The main parameters of the D-μSPE process that influence the extraction (i.e. the amount of sorbent, elution condition, extraction time, and pH) were investigated and optimized. Based on the outputs, the presence of MCC on the surface of MOF-199 leads to improve the properties of MOF-199 and the MCC/MOF-199 has the highest sorption capacity, durability, and porosity in comparison with MCC and MOF-199. According to the validation study at the optimized conditions, the linearity for the analytes was achieved in the range from 0.1 to 200 ng mL⁻¹ for 2-CP and 4-CP and 0.15 to 200 ng mL⁻¹ for 2,3-DCP and 2,5-DCP with correlation coefficients between 0.9928 and 0.9965. The limits of detection calculated at S/N=3 were in the range of 0.03-0.05 ng mL⁻¹. Besides, the relative standard deviations (RSDs) for three spiking levels (0.2, 10,100 ng mL⁻¹) do not exceed 6.8% and extraction recoveries are between 81.0% and 88.3%. Finally, the D-μSPE-HPLC-UV method was successfully applied to the analysis of CPs in real water samples (mineral, river and wastewater samples) with good recoveries (95.8 to 99.5%) and satisfactory precisions (RSD < 6.8%).

在这项研究中，微晶纤维素/金属有机骨架199杂化物（MCC / MOF-199）被用作吸附剂用于氯酚的分散微固相萃取（D-μSPE）。D-μSPE方法与高效液相色谱-紫外检测（HPLC-UV）结合用于测定四种氯酚，包括2-氯酚（2-CP），4-氯酚（4-CP），2,3-水溶液中的二氯苯酚（2,3-DCP）和2,5-二氯苯酚（2,5-DCP）。研究并优化了D-μSPE工艺中影响萃取的主要参数（即吸附剂的量，洗脱条件，萃取时间和pH）。根据输出，MOC-199表面上存在MCC可以改善MOF-199的性能，而MCC / MOF-199具有最高的吸附能力，耐久性，与MCC和MOF-199相比孔隙率更高。根据在最佳条件下进行的验证研究，分析物的线性范围为0.1到200 ng mL^-1 2-CP和4-CP和0.15至200毫微克毫升^-1 2,3- DCP和2,5- DCP与0.9928和0.9965之间的相关系数。在S / N ＝ 3下计算的检测极限在0.03-0.05ngmL ^-1的范围内。此外，三个加标水平（0.2、10,100 ng mL ^-1）的相对标准偏差（RSD）不超过6.8％，提取回收率在81.0％至88.3％之间。最终，D-μSPE-HPLC-UV方法成功地用于实际水样品（矿物，河流和废水样品）中的CPs的回收率（95.8至99.5％）和满意的精度（RSD <6.8％）。