Conventional methods for Yersinia enterocolitica detection in food samples are generally considered inadequate. Problems arise from the presence of the so-called “background flora”, coupled to the low contamination level of the pathogen. Since, data on the microbial ecology occurring in competitive microflora are still lacking, MALDI TOF MS was used for strains ‘identification after enrichment in PSB or ITC broths, and after plating on selective CIN medium at different incubation times. SYBR Green Real time PCR was used for the Y. enterocolitica strains’ detection (4/O:3, 1A/O:5) in experimentally contaminated foods, as well as in naturally contaminated samples. A higher number of different bacterial genera (10 on CIN and 18 on PCA) was recorded after enrichment in PSB, whilst enrichment in ITC led to recovery of 6 and 10 genera on CIN and PCA, respectively. Yersiniaceae was the dominant family on the first day of incubation, but on the second day the percentage of isolation considerably decreased. By testing experimentally contaminated samples, substantial difficulties were encountered. The biotype 1A was always detected, whereas strain 4/O:3 proved to be poorly competitive. Based on the data, the enrichment media PSB and ITC, currently proposed for Y. enterocolitica detection, need to be improved to promote a successful pathogen's recovery.

食品样品中小肠结肠炎耶尔森氏菌的常规检测方法通常被认为是不适当的。问题是由于存在所谓的“背景菌群”，加上病原体的低污染水平。由于仍然缺乏竞争微生物区系中发生的微生物生态学数据，因此将MALDI TOF MS用于富集PSB或ITC肉汤后，以及在不同孵育时间铺板于选择性CIN培养基上后进行菌株鉴定。SYBR Green实时荧光定量PCR用于小肠结肠炎耶尔森氏菌在受实验污染的食物以及自然污染的样品中检测到菌株（4 / O：3、1A / O：5）。PSB富集后，记录了更多数量的不同细菌属（CIN为10，PCA为18），而ITC富集导致CIN和PCA分别恢复了6和10属。在培养的第一天，耶尔森氏菌是优势家族，但是在第二天，分离的百分比大大降低。通过测试实验污染的样品，遇到了很大的困难。始终检测到生物型1A，而菌株4 / O：3证明竞争性很差。根据这些数据，需要改进目前建议用于小肠结肠炎耶尔森氏菌检测的富集培养基PSB和ITC，以促进成功的病原体恢复。