Focus on risks to human health and the environment from combined exposure to multiple chemicals (“mixture risk assessment”) has increased in the last couple of decades. There has been a rise in awareness and concern in the community, especially concerning unintentional environmental exposure to unknown chemical mixtures. The Horizon 2020 project EuroMix has developed methodologies and tools for mixture risk assessment with a focus on component-based approach where substances are grouped based on toxicological considerations. Dose addition is used as the model for calculating the combined toxicity of mixture components. The methodology is anchored in the Adverse Outcome Pathway (AOP) concept, which provides a structured basis for e.g. grouping substances into assessment groups and identifying and collecting relevant toxicity data. The aim of this paper is to describes development of the methodology for mixture risk assessment and to provide detailed methodology for problem formulation, use of AOP networks for development of tiered testing strategies and grouping of substances, as well as considerations for use of dose addition methodology.

在过去的几十年中，人们越来越关注通过多种化学物质的综合接触对人类健康和环境造成的风险（“混合物风险评估”）。社区中的意识和关注度不断提高，尤其是在无意间暴露于未知化学混合物的环境中。Horizo​​n 2020项目EuroMix开发了用于混合物风险评估的方法和工具，重点是基于成分的方法，其中基于毒理学考虑将物质分组。剂量添加用作计算混合物组分的组合毒性的模型。该方法以“不良结果途径”（AOP）概念为基础，该概念为例如将物质分组到评估组以及识别和收集相关毒性数据提供了结构化基础。