Origanum majorana essential oil (OmEO) encapsulated into chitosan nanoemulsion is being reported as a novel preservative of stored food items against fungi, aflatoxin B₁ (AFB₁) contamination and lipid peroxidation. The major component of OmEO identified through GC-MS was terpinen-4-ol (28.92%). HR-SEM, FTIR and XRD analyses confirmed successful encapsulation of OmEO into chitosan nanoemulsion (OmEO-CsNe). The results showed remarkable improvement in efficacy after nanoencapsulation, since OmEO-CsNe completely inhibited the growth and AFB₁ production by Aspergillus flavus at 1.0 μL/mL, which was 2.5 and 1.5 μL/mL, respectively for OmEO. The inhibition of ergosterol followed by release of cellular ions and 260 and 280 nm absorbing materials demonstrated plasma membrane as possible antifungal target. Inhibition of methylglyoxal confirmed antiaflatoxigenic mode of action. OmEO-CsNe showed enhanced antioxidant activity (IC₅₀ = 14.94 and 5.53 μL/mL for DPPH and ABTS, respectively) and caused in situ inhibition of lipid peroxidation and AFB₁ production in maize (third most important staple crop after wheat and rice) without altering their sensory attributes and presented safety profile (LD₅₀ = 11,889 μL/kg) when tested on mice. The findings indicate that the encapsulation considerably enhances the performance of OmEO, therefore can be recommended as a promising antifungal agent to extend the shelf-life of food items.

据报道，封装在壳聚糖纳米乳液中的牛至精油（Om EO）是一种新型食品防腐剂，可防止真菌，黄曲霉毒素B ₁（AFB ₁）污染和脂质过氧化作用。通过GC-MS鉴定出的Om EO的主要成分为萜品四醇（28.92％）。HR-SEM，FTIR和XRD分析证实Om EO成功地封装到壳聚糖纳米乳液（Om EO-CsNe）中。结果显示纳米封装后功效显着提高，因为Om EO-CsNe完全抑制了黄曲霉的生长和AFB _1的产生浓度为1.0μL / mL，对于Om EO分别为2.5和1.5μL/ mL 。麦角固醇的抑制作用随后释放细胞离子以及260和280 nm的吸收材料证明质膜是可能的抗真菌靶标。抑制甲基乙二醛证实了抗黄曲霉毒素的作用方式。Om EO-CsNe表现出增强的抗氧化活性（ DPPH和ABTS的IC _50分别为14.94和5.53μL / mL），并引起玉米中脂质过氧化和AFB ₁产生的原位抑制（仅次于小麦和水稻的第三重要农作物）而不改变其感官属性和安全性（LD ₅₀ = 11,889μL/ kg）。研究结果表明，这种封装大大提高了Om EO的性能，因此可以推荐作为一种有希望的抗真菌剂来延长食品的保质期。