An innovative sandwich MIP-based electrochemical sensor was fabricated for the fast and selective detection of adenosine. The covalent-imprinted biomimetic recognition element relies on two overlapping polymeric films, an underlaying composite boronate-affinity and poly(bithiophene)-structural layer and an overlaying poly(3-indolacetic acid) coating bearing carboxyl groups, intended to further shape the selectivity of the sensor towards adenosine. The sensor’s behavior and analytical performance was characterized by cyclic voltammetry (CV), differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS). The recorded DPVs showed a linear response to increasing concentration of adenosine in the range of 0.37 μM–37.4 μM with a limit of detection of 0.21 μM. The selectivity of the MIP sensor towards adenosine was demonstrated against more than 10 structurally related interferents. The developed sensor was applied for the fast and quantitative assessment of adenosine in spiked urine samples with good recoveries (102.98%–106.34%), proving at least comparable bioanalytical performances in terms of sensitivity, yet greatly improved in terms of selectivity and analysis time to previously reported sensors.

制造了一种基于三明治式MIP的创新型电化学传感器，用于快速，选择性地检测腺苷。共价印迹仿生识别元件依赖于两个重叠的聚合物膜，一个底层复合硼酸酯亲和力和聚（联噻吩）结构层以及一个带有羧基的覆盖聚（3-吲哚乙酸）涂层，旨在进一步塑造聚乙烯吡咯烷酮的选择性。朝向腺苷的传感器。通过循环伏安法（CV），差分脉冲伏安法（DPV）和电化学阻抗谱（EIS）表征了传感器的行为和分析性能。记录的DPV显示在0.37 μM–37.4μM范围内对腺苷浓度增加具有线性响应，检出限为0.21μM。证明了MIP传感器对腺苷的选择性针对10多种与结构相关的干扰物。研发的传感器用于快速定量定量加标的尿液样品中的腺苷，回收率良好（102.98％–106.34％），在灵敏度方面至少证明了可比的生物分析性能，但在选择性和分析时间方面大大改善了先前报告的传感器。