In this study, Pseudomonas aeruginosa was applied to degrade tetrabromobisphenol A (TBBPA) with glucose as a co-metabolic substrate. Influencing factors of co-metabolic degradation such as pH, TBBPA and glucose concentration were examined and the degradation efficiency under optimal condition reached about 50% on the 7th day. The study also proved that the extracellular action, rather than intracellular one, played a leading role in TBBPA degradation. Five metabolites including debromination and beta-scission products were identified in this study. The extracellular active substance pyocyanin was considered as the origin of H₂O₂ and OH·. The variation of concentrations of H₂O₂ and OH· shared the same trend, they increased in the early days and then declined gradually. On the 1st day, the OD600 of P.aeruginosa in the co-metabolic group was 6.0 times higher than the initial value while total organic carbon (TOC) decreased about 78%, which might lead to the occurrence of pyocyanin auto-poisoning. Flow cytometry was applied to detect the cellular state of P.aeruginosa during degradation. The increasing intracellular ROS showed that cells were suffering from oxidative stress and the change of membrane potential revealed that cellular dysfunction had occurred since the 1st day. This research indicated that the toxic effect on P.aeruginosa was probably not directly correlated with TBBPA, but was caused by pyocyanin auto-poisoning.

在这项研究中，铜绿假单胞菌被用于降解四溴双酚A（TBBPA），其中葡萄糖为共代谢底物。研究了代谢代谢的影响因素，如pH，TBBPA和葡萄糖浓度，在最佳条件下第7天的降解效率达到了约50％。该研究还证明，细胞外作用而不是细胞内作用在TBBPA降解中起主导作用。在这项研究中鉴定出包括脱溴和β-分裂产物在内的五种代谢物。细胞外活性物质黄绿素被认为是H ₂ O ₂和OH·的起源。H ₂ O ₂的浓度变化OH·和OH·的趋势相同，它们在开始时先上升然后逐渐下降。第一天，代谢组中的绿脓杆菌的OD600是初始值的6.0倍，而总有机碳（TOC）下降了约78％，这可能导致黄绿素自动中毒的发生。流式细胞仪用于检测降解过程中铜绿假单胞菌的细胞状态。细胞内ROS的增加表明细胞正遭受氧化应激，膜电位的变化表明细胞自第一天起就发生了功能障碍。这项研究表明对铜绿假单胞菌的毒性作用 可能与TBBPA没有直接关系，但是由黄绿素自动中毒引起的。