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Heterologous expression of lytic polysaccharide monooxygenases (LPMOs).
Biotechnology Advances ( IF 16.0 ) Pub Date : 2020-07-04 , DOI: 10.1016/j.biotechadv.2020.107583
Yasser Gaber 1 , Boshra Rashad 2 , Rasha Hussein 3 , Mai Abdelgawad 2 , Nourhan S Ali 2 , Tarek Dishisha 4 , Anikó Várnai 5
Affiliation  

Lytic polysaccharide monooxygenases (LPMOs) are relatively new enzymes that have been discovered 10 years ago. LPMOs comprise a diverse group of enzymes which play a pivotal role in the depolymerization of sugar-based biopolymers including cellulose, hemicellulose, chitin, and starch. Their mechanism of action relies on the correct coordination of a copper ion in the active site, which is partly composed of the N-terminal histidine. Therefore, correct secretion and folding of these copper-enzymes is fundamental for obtaining a catalytic activity. LPMOs occur in all kingdoms of life; they have been found in viruses, bacteria and eukaryotes, including fungi, plants and animals. In many cases, using homologous expression of these proteins is not feasible and an alternative organism, which can be cultured and is able to heterologously express the protein of interest, is required for studying enzyme properties. Therefore, we made an extensive compilation of expression techniques used for LPMOs the expression and characterization of which have been reported to date. In the current review, we provide a summary of the different techniques, including expression hosts and vectors, secretion methods, and culturing conditions, that have been used for the overexpression and production of this important class of enzymes at laboratory scale. Herein, we compare these techniques and assess their advantages and disadvantages.



中文翻译:

溶解性多糖单加氧酶(LPMO)的异源表达。

裂解多糖单加氧酶(LPMO)是10年前发现的相对较新的酶。LPMO包含多种酶,这些酶在糖基生物聚合物(包括纤维素,半纤维素,甲壳质和淀粉)的解聚中起关键作用。它们的作用机理依赖于活性部位中铜离子的正确配位,该活性部位部分由N端组氨酸组成。因此,这些铜酶的正确分泌和折叠对于获得催化活性至关重要。LPMOs出现在所有生活王国中;它们已在病毒,细菌和真核生物(包括真菌,植物和动物)中发现。在许多情况下,使用这些蛋白质的同源表达是不可行的,因此,替代生物 为了研究酶的特性,需要将其培养并能够异源表达目的蛋白。因此,我们对用于LPMO的表达技术进行了广泛的汇编,迄今为止已经报道了其表达和表征。在当前的综述中,我们提供了不同技术的摘要,包括表达宿主和载体,分泌方法和培养条件,这些技术已用于在实验室规模过表达和生产这种重要的酶类。在这里,我们比较这些技术并评估它们的优缺点。在当前的综述中,我们提供了不同技术的摘要,包括表达宿主和载体,分泌方法和培养条件,这些技术已用于在实验室规模过表达和生产这种重要的酶类。在这里,我们比较这些技术并评估它们的优缺点。在当前的综述中,我们提供了不同技术的摘要,包括表达宿主和载体,分泌方法和培养条件,这些技术已用于在实验室规模过表达和生产这种重要的酶类。在这里,我们比较这些技术并评估它们的优缺点。

更新日期:2020-07-04
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