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Efficacy of the essential oils of Mentha piperita, Lippia alba and Zingiber officinale to control the acanthocephalan Neoechinorhynchus buttnerae in Colossoma macropomum
Aquaculture Reports ( IF 3.7 ) Pub Date : 2020-07-04 , DOI: 10.1016/j.aqrep.2020.100414
Carinne Moreira de Souza Costa , Matheus Gomes da Cruz , Talissa Beatriz Costa Lima , Liliane Campos Ferreira , Arlene Sobrinho Ventura , Franmir Rodrigues Brandão , Edsandra Campos Chagas , Francisco Célio Maia Chaves , Maurício Laterça Martins , Gabriela Tomas Jerônimo

This study evaluated the in vitro and in vivo parasiticidal efficacy of the essential oils of Mentha piperita, Lippia alba and Zingiber officinale on Neoechinorhynchus buttnerae (acanthocephalan) and their effect when used as dietary supplementation on hematological parameters of Colossoma macropomum. For the in vitro assay, the parasites were exposed to five different concentrations (360, 540, 720, 1440, and 2880 mg L−1) of the essential oils diluted in 3% Tween 80, for incubation period of 24 hs observed each 15 min. The essential oil of M. piperita caused mortality of 100 % of the parasites in 2 h in the concentration (540 mg L−1), compared to the essential oils of L. alba and Z. officinale. For the in vivo tests, 210 juvenile tambaqui were used; they were fed for 30 days with either the control diet and experimental diets consisting of supplementation with essential oils: control, M. piperita 0.54 g kg−1 and 2.88 g kg−1, L. alba 1.44 g kg-1 and 2.88 g kg−1, Z. officinale 1.44 g kg−1 and 2.88 g kg−1, in triplicate, resulting in 21 experimental units (10 fish per tank). The anthelmintic activity in the animals treated using diets supplemented with M. piperita at 0.54 and 2.88 g kg−1 were 85.46 % and 70.03 %; with L. alba 1.44 and 2.88 g kg−1 were 67.10 % and 56.93 %, and with Z. officinale 1.44 and 2.88 g kg−1 were 11.70 % and 5.19 %, respectively. Total red blood cells count and thrombocytes were higher in fish treated with M. piperita at 0.54 g kg−1 and Z. officinale at 1.44 g kg−1. The supplementation with essential oil of M. piperita at 0.54 g kg−1 was effective in the control of N. buttnerae and presented better specific growth rate. However, despite these promising results, more research is needed to validate their use in commercial production.



中文翻译:

的精油功效薄荷马鞭草阿尔巴生姜来控制棘Neoechinorhynchus buttnerae淡水白macropomum

这项研究评估了薄荷(Mentha piperita)白头翁(Lippia alba)生姜Zingiber officinale)精油对新棘皮hy(acanthocephalan)的体外体内杀虫效果,以及当其作为膳食补充剂对巨像(Colossoma macropomum)血液学参数的影响。对于体外测定,将寄生虫暴露于稀释在3%Tween 80中的五种不同浓度(360、540、720、1440和2880 mg L -1)的香精油中,每15观察到24小时的孵育时间分钟 M. piperita的精油L. albaZ. officinale的香精油相比,在2 h浓度(540 mg L -1)中引起100%的寄生虫死亡。对于体内试验,210少年坦巴基分别使用; 他们分别用对照饮食和实验饮食喂养30天,实验饮食包括精油补充:对照,胡椒分枝杆菌0.54 g kg -1和2.88 g kg -1白叶乳杆菌1.44 g kg -1和2.88 g kg -1,山茱Z 1.44 g kg -1和2.88 g kg -1,一式三份,产生21个实验单位(每槽10条鱼)。在添加了0.54和2.88 g kg -1的胡椒支原体的日粮中治疗的动物的驱虫活性为85.46%和70.03%;与L.粉1.44和2.88g千克-1分别为67.10%和56.93%,且与Z.铁皮1.44和2.88g千克-1分别为11.70%和5.19%。总红细胞计数和血小板都与处理的鱼更高M. piperita在0.54克千克-1Z.铁皮在1.44克千克-1。以0.54 g公斤补充胡椒分枝杆菌的精油-1在控制猪笼草中很有效,并且比生长速率更高。然而,尽管取得了这些令人鼓舞的结果,但仍需要进行更多的研究以验证其在商业生产中的使用。

更新日期:2020-07-04
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