Since less than a decade ago, the DNA origami technique has become an important tool in nanopore fabrication. DNA origami nanopores are highly efficient because of their compatibility with biomolecules and the possibility to precisely engineer their dimensions and designs. However, accurate comprehension of their molecular behavior under various conditions is still unsatisfactory. In this study, a thin plate DNA origami nanopore is designed and investigated using molecular dynamics simulation. The thin plate is designed using caDNAno software along with the square lattice method and the molecular dynamics simulation is performed using GROMACS software. The model is simulated in a wide temperature range and its stability is investigated. The shape and dimensions of the nanopore are also compared at these temperatures. The results indicate that the designed nanopore exhibits decent stability at these temperatures and no breakdown was observed despite some distortions in the structure at high temperatures. In addition, the effect of the number of staple strands on the structure, stability, and deformation of the DNA origami plate is investigated and it is found that addition of staple strands have a significant positive effect on the stability of nanopore’s shape. By the results of analyzing the shape of the nanopore, it suggests that the proposed nanopore can be used to pass a wide range of molecules, macromolecules, and drug cargoes.

自不到十年前以来，DNA折纸技术已成为纳米孔制造中的重要工具。DNA折纸纳米孔具有很高的效率，因为它们与生物分子具有相容性，并且可以精确设计其尺寸和设计。但是，仍然不能令人满意地准确理解它们在各种条件下的分子行为。在这项研究中，设计了一种薄板DNA折纸纳米孔，并使用分子动力学模拟进行了研究。使用caDNAno软件以及方格方法设计薄板，并使用GROMACS软件进行分子动力学模拟。该模型在较宽的温度范围内进行了仿真，并研究了其稳定性。在这些温度下还比较了纳米孔的形状和尺寸。结果表明，设计的纳米孔在这些温度下表现出不错的稳定性，尽管在高温下结构发生了一些变形，但未观察到击穿现象。另外，研究了短链的数量对DNA折纸板的结构，稳定性和变形的影响，发现短链的添加对纳米孔形状的稳定性具有显着的积极影响。通过分析纳米孔的形状的结果，表明所提出的纳米孔可用于传递各种分子，大分子和药物货物。研究了DNA折纸板的稳定性和变形，发现添加订书钉链对纳米孔形状的稳定性具有显着的积极影响。通过分析纳米孔的形状的结果，表明所提出的纳米孔可用于传递各种分子，大分子和药物货物。研究了DNA折纸板的稳定性和变形，发现添加订书钉链对纳米孔形状的稳定性具有显着的积极影响。通过分析纳米孔的形状的结果，表明所提出的纳米孔可用于传递各种分子，大分子和药物货物。