Purification and characterization of glucoamylase of Aspergillus oryzae from Luzhou-flavour Daqu,Biotechnology Letters

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Purification and characterization of glucoamylase of Aspergillus oryzae from Luzhou-flavour Daqu
Biotechnology Letters ( IF 2.7 ) Pub Date : 2020-07-04 , DOI: 10.1007/s10529-020-02956-4
Chuan Wang ₁ , Lianli Yang ₁ , Lunan Luo ₁ , Shichao Tang ₁ , Qiang Wang ₁

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To obtain novel glucoamylase from Daqu microbe. A dominant strain known as LZ2 with high activity of hydrolyzing starch was isolated from Luzhou Daqu, a Chinese traditional fermentation starter. The LZ2 was identified as Aspergillus oryzae by 18S rDNA sequence analysis. Glucoamylase from LZ2, named as GA-LZ2, was purified to homogeneity and showed a single band with expected molecular mass of 60 kD. The GA-LZ2 effectively degraded amylose, rice starch and wheat starch. Optimal temperature and pH value of enzyme were 60 °C and pH 4.0 respectively. The GA-LZ2 displayed significant thermal stability and pH stability at moderate temperature and low pH. Intriguingly, the thermostability was enhanced in the presence of starch. In addition, GA-LZ2 exhibited insensitivity to glucose, independence of metal ions and tolerance to organic solvents. The GA-LZ2 retained complete activity in the presence of 100 mM glucose and 5% ethanol and methanol. Glucoamylase GA-LZ2 displayed broad substrate specificity, strong stability and tolerance, suggesting that GA-LZ2 carry potential for industrial application in bioethanol production.

更新日期：2020-07-04

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