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Valorization of apple pomace using bio-based technology for the production of xylitol and 2G ethanol.
Bioprocess and Biosystems Engineering ( IF 3.8 ) Pub Date : 2020-07-05 , DOI: 10.1007/s00449-020-02401-w
Lillian Vieira Leonel 1 , Luciane Sene 1 , Mário Antônio Alves da Cunha 2 , Kátia Caroline França Dalanhol 3 , Maria das Graças de Almeida Felipe 3
Affiliation  

Apple pomace was studied as a raw material for the production of xylitol and 2G ethanol, since this agroindustrial residue has a high concentration of carbohydrate macromolecules, but is still poorly studied for the production of fermentation bioproducts, such as polyols. The dry biomass was subjected to dilute-acid hydrolysis with H2SO4 to obtain the hemicellulosic hydrolysate, which was concentrated, detoxified and fermented. The hydrolyzate after characterization was submitted to submerged fermentations, which were carried out in Erlenmeyer flasks using, separately, the yeasts Candida guilliermondii and Kluyveromyces marxianus. High cellulose (32.62%) and hemicellulose (23.60%) contents were found in this biomass, and the chemical hydrolysis yielded appreciable quantities of fermentable sugars, especially xylose. Both yeasts were able to metabolize xylose, but Candida guilliermondii produced only xylitol (9.35 g L−1 in 96 h), while K.marxianus produced ethanol as the main product (10.47 g L−1 in 24 h) and xylitol as byproduct (9.10 g L−1 xylitol in 96 h). Maximum activities of xylose reductase and xylitol dehydrogenase were verified after 24 h of fermentation with C.guilliermondii (0.23 and 0.53 U/mgprot, respectively) and with K.marxianus (0.08 e 0.08 U/mgprot, respectively). Apple pomace has shown potential as a raw material for the fermentation process, and the development of a biotechnological platform for the integrated use of both the hemicellulosic and cellulosic fraction could add value to this residue and the apple production chain.



中文翻译:

使用生物基技术生产木糖醇和 2G 乙醇对苹果渣进行增值。

苹果渣被研究作为生产木糖醇和 2G 乙醇的原料,因为这种农业工业残留物含有高浓度的碳水化合物大分子,但在生产发酵生物产品(如多元醇)方面的研究仍然很少。干生物质经H 2 SO 4稀酸水解得到半纤维素水解物,经浓缩、解毒、发酵。表征后的水解产物进行深层发酵,该发酵在锥形瓶中进行,分别使用酵母念珠菌马克斯克鲁酵母. 在这种生物质中发现了高纤维素 (32.62%) 和半纤维素 (23.60%) 含量,化学水解产生了大量的可发酵糖,尤其是木糖。两个酵母菌能够代谢木糖,但念珠菌季也蒙只产生木糖醇(9.35克L- -1在96小时),而K.维酵母产生乙醇作为主要产物(10.47克L- -1在24小时)和木糖醇作为副产物( 9.10 g L -1木糖醇在 96 小时内)。木糖还原酶和木糖醇脱氢酶的最大活性在用C. guilliermondii(分别为 0.23 和 0.53 U/mg prot)和K发酵 24 小时后得到验证marxianus(分别为 0.08 e 0.08 U/mg prot)。苹果渣已显示出作为发酵过程原材料的潜力,开发一个生物技术平台来综合利用半纤维素和纤维素部分,可以为这种残留物和苹果生产链增加价值。

更新日期:2020-07-05
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