In this study, we prepared oligodeoxynucleotides (ODNs) containing the uridine base modified by an alkyl chain at the 5-position (^AU) and characterized their aggregate formation, localization, and functions in cells. These experiments revealed that aggregates of these ODNs were readily transported into cells, but their localization was dependent upon the number of hydrophobic units. ODNs with one modified ^AU were transported in the cytosol, while ODNs with multiple ^AU modifications resulted in their accumulation at the cell membrane. We also examined the ability of the ^AU-modified ODNs to capture small molecules at the cell membrane and their cellular uptake. We positioned a thioflavin-T (ThT)-binding aptamer on the cell membrane by means of hybridization with ODNs with three ^AUs at the strand end. Treatment with ThT resulted in its efficient uptake into cells, due to the capture of the ThT by the aptamers on the cell membrane. Thus, we demonstrated the functionalization of cell membranes with modified ODNs and the efficient delivery of small molecules into the cells.

中文翻译：

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用含有烷基链修饰的尿苷的寡聚脱氧核苷酸的聚集体调节细胞膜功能化。

在这项研究中，我们制备了包含在5位（^A U）上被烷基链修饰的尿苷碱基的寡聚脱氧核苷酸（ODN），并表征了它们在细胞中的聚集形成，定位和功能。这些实验表明，这些ODN的聚集体很容易转运到细胞中，但是它们的定位取决于疏水单元的数量。具有一个修饰的^A U的ODN在细胞质中运输，而具有多个^A U修饰的ODN导致它们在细胞膜上积累。我们还检查了^A的能力U修饰的ODN可以捕获细胞膜上的小分子并吸收细胞。我们通过与在链末端具有三个^A Us的ODN杂交，将硫黄素-T（ThT）结合适体定位在细胞膜上。由于ThT被细胞膜上的适体捕获，因此用ThT处理可有效吸收细胞。因此，我们证明了具有修饰的ODN的细胞膜功能化以及小分子向细胞内的有效传递。