当前位置: X-MOL 学术BMC Plant Biol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Genetic analysis of loose smut (Ustilago tritici) resistance in Sonop spring wheat.
BMC Plant Biology ( IF 5.3 ) Pub Date : 2020-07-03 , DOI: 10.1186/s12870-020-02525-x
Dinushika Thambugala 1 , Jim G Menzies 1 , Ron E Knox 2 , Heather L Campbell 2 , Curt A McCartney 1
Affiliation  

The genetics of resistance to loose smut of wheat (Triticum aestivum L.) caused by the fungus Ustilago tritici (Pers.) Rostr. is not well understood. This study examines loose smut resistance in Sonop (TD-14), a South African spring wheat variety. A doubled haploid (DH) population of 163 lines derived from the cross Diamont/TD-14 was studied. The parents and progenies were inoculated with U. tritici races T2, T9, and T39 individually in growth facilities at Morden and Swift Current, Canada. Loose smut incidence (LSI) and partial loose smut resistance (PLSR) were assessed. A whole genome linkage map was developed consisting of 11,519 SNP loci found on 31 linkage groups spanning 2845 cM. A new major resistance gene Ut11 was located to the distal end of chromosome arm 7BS. Ut11 conferred resistance to U. tritici race T2, but not races T9 and T39. Quantitative trait locus (QTL) mapping identified four QTL controlling LSI in the Diamont/TD-14 DH population on chromosomes 3B, 4B, 5B, and 7B (at Ut11) with TD-14 contributing the resistance alleles at three of these loci. The major QTL QUt.mrc-5B was effective against all three races and explained up to 81% of the phenotypic variation. The only QTL identified for PLSR coincided with the LSI QTL QUt.mrc-5B indicating that this locus affected both loose smut incidence and partial smutting of spikes. A race-specific resistance gene Ut11 and a broadly effective resistance QTL QUt.mrc-5B were the main loci controlling loose smut resistance in the differential line TD-14 (cultivar Sonop). This study provides insight into the genetics of loose smut resistance in spring wheat Sonop and the single nucleotide polymorphism (SNP) markers linked to the resistance gene Ut11 and QTL QUt.mrc-5B will be useful for selecting loose smut resistance in breeding programs.

中文翻译:

Sonop春小麦抗黑曲病(Ustilago tritici)的遗传分析。

小麦Ustilago tritici(Pers。)Rostr引起的小麦散发黑穗病的抗性遗传学。尚未完全了解。这项研究研究了南非春小麦品种Sonop(TD-14)的抗黑穗病。研究了来自十字Diamont / TD-14的163个品系的双倍单倍体(DH)群体。亲本和后代分别在加拿大莫登和斯威夫特潮流的生长设施中接种了U. tritici种族T2,T9和T39。评估了黑穗病发病率(LSI)和部分黑穗病抗性(PLSR)。建立了一个全基因组连锁图谱,该图谱由在跨越2845 cM的31个连锁组中发现的11,519个SNP位点组成。一个新的主要抗性基因Ut11位于染色体臂7BS的末端。Ut11赋予了对U. tritici种族T2的抵抗力,但没有抵抗T9和T39种族。数量性状基因座(QTL)定位图在3B,4B,5B和7B号染色体(在Ut11处)的Diamont / TD-14 DH群体中鉴定出四个QTL控制LSI,其中TD-14在这三个基因座中贡献了抗性等位基因。主要的QTL QUt.mrc-5B对所有三个种族均有效,并解释了高达81%的表型变异。唯一为PLSR鉴定的QTL与LSI QTL QUt.mrc-5B吻合,表明该基因座既影响散发的黑穗病发病率,也影响尖峰的部分黑穗病。种族特异性抗性基因Ut11和广泛有效的抗性QTL QUt.mrc-5B是控制差异系TD-14(品种Sonop)中的松散黑穗病抗性的主要基因座。
更新日期:2020-07-03
down
wechat
bug