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A quaternary ammonium silane antimicrobial triggers bacterial membrane and biofilm destruction.
Scientific Reports ( IF 4.6 ) Pub Date : 2020-07-03 , DOI: 10.1038/s41598-020-67616-z
Umer Daood 1 , Jukka P Matinlinna 2 , Malikarjuna Rao Pichika 3 , Kit-Kay Mak 3 , Venkateshbabu Nagendrababu 1 , Amr S Fawzy 4
Affiliation  

To study the antimicrobial effects of quaternary ammonium silane (QAS) exposure on Streptococcus mutans and Lactobacillus acidophilus bacterial biofilms at different concentrations. Streptococcus mutans and Lactobacillus acidophilus biofilms were cultured on dentine disks, and incubated for bacterial adhesion for 3-days. Disks were treated with disinfectant (experimental QAS or control) and returned to culture for four days. Small-molecule drug discovery-suite was used to analyze QAS/Sortase-A active site. Cleavage of a synthetic fluorescent peptide substrate, was used to analyze inhibition of Sortase-A. Raman spectroscopy was performed and biofilms stained for confocal laser scanning microscopy (CLSM). Dentine disks that contained treated dual-species biofilms were examined using scanning electron microscopy (SEM). Analysis of DAPI within biofilms was performed using CLSM. Fatty acids in bacterial membranes were assessed with succinic-dehydrogenase assay along with time-kill assay. Sortase-A protein underwent conformational change due to QAS molecule during simulation, showing fluctuating alpha and beta strands. Spectroscopy revealed low carbohydrate intensities in 1% and 2% QAS. SEM images demonstrated absence of bacterial colonies after treatment. DAPI staining decreased with 1% QAS (p < 0.05). Fatty acid compositions of dual specie biofilm increased in both 1% and 2% QAS specimens (p < 0.05). Quaternary ammonium silane demonstrated to be a potent antibacterial cavity disinfectant and a plaque inhibitor and can be of potential significance in eliminating caries-forming bacteria.



中文翻译:

季铵硅烷抗菌剂会触发细菌膜和生物膜破坏。

研究季铵硅烷(QAS)暴露对变形链球菌嗜酸乳杆菌细菌生物膜在不同浓度下的抗菌作用。变形链球菌嗜酸乳杆菌生物膜在牙本质盘上培养,并温育细菌粘附3天。圆盘用消毒剂(实验性QAS或对照)处理,并培养4天。使用小分子药物发现套件来分析QAS / Sortase-A活性位点。合成荧光肽底物的切割用于分析Sortase-A的抑制。进行拉曼光谱分析,并对生物膜染色,以进行共聚焦激光扫描显微镜检查(CLSM)。使用扫描电子显微镜(SEM)检查包含处理过的双物种生物膜的牙本质盘。使用CLSM对生物膜中的DAPI进行分析。用琥珀酸脱氢酶测定法和时间杀灭测定法评估细菌膜中的脂肪酸。在模拟过程中,由于QAS分子的作用,Sortase-A蛋白发生了构象变化,显示波动的α和β链。光谱显示在1%和2%QAS中碳水化合物含量低。SEM图像显示处理后不存在细菌菌落。DAPI染色随1%QAS降低(p  <0.05)。在1%和2%的QAS标本中,双物种生物膜的脂肪酸组成均增加(p  <0.05)。季铵硅烷被证明是有效的抗菌腔体消毒剂和噬菌斑抑制剂,在消除龋齿形成细菌方面可能具有重要意义。

更新日期:2020-07-03
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