Ectoine, a compatible solute synthesized by many halophiles for hypersalinity resistance, has been successfully produced by metabolically engineered Halomonas bluephagenesis, which is a bioplastic poly(3-hydroxybutyrate) producer allowing open unsterile and continuous conditions. Here we report a de novo synthesis pathway for ectoine constructed into the chromosome of H. bluephagenesis utilizing two inducible systems, which serve to fine-tune the transcription levels of three clusters related to ectoine synthesis, including ectABC, lysC and asd based on a GFP-mediated transcriptional tuning approach. Combined with bypasses deletion, the resulting recombinant H. bluephagenesis TD-ADEL-58 is able to produce 28 g L⁻¹ ectoine during a 28 h fed-batch growth process. Co-production of ectoine and PHB is achieved to 8 g L⁻¹ ectoine and 32 g L⁻¹ dry cell mass containing 75% PHB after a 44 h growth. H. bluephagenesis demonstrates to be a suitable co-production chassis for polyhydroxyalkanoates and non-polymer chemicals such as ectoine.

Ectoine是一种由许多嗜盐菌合成的具有相容性的溶质，可抵抗高盐度，它是通过代谢工程化的Halomonas bluephagenesis成功生产的，Halomonas bluephagenesis是一种生物塑料聚（3-羟基丁酸酯）生产商，允许在开放的不育和连续条件下生产。在这里，我们报告利用两个可诱导系统构建的蓝藻H.bluephagenesis染色体中的植物素的从头合成途径，其用于微调与植物素合成相关的三个簇的转录水平，包括基于GFP的ectABC，lysC和asd介导的转录调节方法。与旁路缺失相结合，产生重组的蓝细菌TD-ADEL-58能够在28小时的分批补料生长过程中产生28 g L ^-1的植物素。生长44小时后，可将8g L ^-1 ectoine和32 g L ^-1的干细胞（含75％PHB）联合生产ectoine和PHB 。H. bluephagenesis已证明是适用于聚羟基链烷酸酯和非聚合物化学物质（如ectoine）的联产底盘。