Solid-state nanopores provide a highly sensitive tool for single-molecule sensing and probing nanofluidic effects in solutions. Glass nanopipettes are a cheap and robust type of solid-state nanopore produced from pulling glass capillaries with opening orifice diameters down to below tens of nanometers. Sub-50 nm nanocapillaries allow an unprecedented resolution for translocating single molecules or for scanning ion conductance microscopy imaging. Due to the small opening orifice diameters, such nanocapillaries are difficult to fill with solutions, compromising their advantages of low cost, availability, and experimental simplicity. We present a simple and cheap method to reliably fill nanocapillaries down to sub-10 nm diameters by microwave radiation heating. Using a large statistic of filled nanocapillaries, we determine the filling efficiency and physical principle of the filling process using sub-50 nm quartz nanocapillaries. Finally, we have used multiple nanocapillaries filled by our method for high-resolution scanning ion conductance microscopy imaging.

中文翻译：

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微波辐射实现高通量纳米毛细管填充，用于扫描离子电导显微镜成像

固态纳米孔为单分子传感和探测溶液中的纳米流体效应提供了高度灵敏的工具。玻璃纳米移液器是一种廉价而坚固的固态纳米孔，它是通过拉开开口直径降至数十纳米以下的玻璃毛细管而产生的。50 nm以下的纳米毛细管为单分子移位或扫描离子电导显微镜成像提供了前所未有的分辨率。由于开孔直径小，此类纳米毛细管很难用溶液填充，从而损害了它们的低成本，实用性和实验简便性的优势。我们提出了一种简单便宜的方法，通过微波辐射加热可靠地填充直径小于10 nm的纳米毛细管。使用大量的填充纳米毛细管统计数据，我们使用低于50 nm的石英纳米毛细管来确定填充过程的填充效率和物理原理。最后，我们已经使用通过我们的方法填充的多个纳米毛细管进行高分辨率扫描离子电导显微镜成像。