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Rapid activation of distinct members of multigene families in Plasmodium spp.
Communications Biology ( IF 5.9 ) Pub Date : 2020-07-03 , DOI: 10.1038/s42003-020-1081-3
Radoslaw Igor Omelianczyk 1 , Han Ping Loh 1 , Marvin Chew 1, 2 , Regina Hoo 1 , Sebastian Baumgarten 3 , Laurent Renia 4 , Jianzhu Chen 2, 5 , Peter R Preiser 1, 2
Affiliation  

The genomes of Plasmodium spp. encode a number of different multigene families that are thought to play a critical role for survival. However, with the exception of the P. falciparum var genes, very little is known about the biological roles of any of the other multigene families. Using the recently developed Selection Linked Integration method, we have been able to activate the expression of a single member of a multigene family of our choice in Plasmodium spp. from its endogenous promoter. We demonstrate the usefulness of this approach by activating the expression of a unique var, rifin and stevor in P. falciparum as well as yir in P. yoelii. Characterization of the selected parasites reveals differences between the different families in terms of mutual exclusive control, co-regulation, and host adaptation. Our results further support the application of the approach for the study of multigene families in Plasmodium and other organisms.



中文翻译:

疟原虫中多基因家族不同成员的快速激活。

疟原虫的基因组。编码许多不同的多基因家族,这些家族被认为对生存至关重要。但是,除了恶性疟原虫var基因外,对其他任何多基因家族的生物学作用知之甚少。使用最近开发的选择链接整合方法,我们已经能够激活疟原虫属物种中我们选择的多基因家族的单个成员的表达。从其内源启动子。我们通过激活一种独特的表达,证明这种方法的有用变种,rifinSTEVOR恶性疟原虫以及约氏疟原虫。所选寄生虫的特征揭示了在相互排斥控制,共同调控和宿主适应性方面不同家族之间的差异。我们的结果进一步支持了该方法在疟原虫和其他生物中研究多基因家族的应用。

更新日期:2020-07-03
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