Despite its great potential in cancer therapy, phototherapy, including photothermal therapy (PTT) and photodynamic therapy (PDT), often cause metastasis of tumors. Immunotherapy has revolutionized the cancer treatment owing to the capability of activating immune system to eliminate tumors. However, the integration of phototherapy and immunotherapy in a single nanoagent for cancer therapy is still a challenging task. Here, we fabricated (Cu₉S₅@mSiO₂-PpIX@MnO₂@CpG (CSPM@CpG)) as a synergistic therapeutic model for phototherapy enhanced immunotherapy. The intracellular uptake of cytosine-phosphate-guanine (CpG) promoted the infiltration of cytotoxic T lymphocytes (CTLs) in tumor tissue, further stimulating the production of interferon gamma (IFN-γ) and remarkably elevating the immune response level. Excellent anti-tumor effects have been achieved by synergistic PTT/PDT/immunotherapy. The metastasis of tumors was effectively inhibited by the immune response of CpG. Thus, our proposed work provides a strategy to combine phototherapy with immunotherapy to enhance the therapeutic efficiency and further inhibit metastasis of tumors.

尽管光疗法在癌症治疗中具有巨大潜力，但是光疗法，包括光热疗法（PTT）和光动力疗法（PDT），通常会导致肿瘤转移。由于激活免疫系统消除肿瘤的能力，免疫疗法彻底改变了癌症治疗方法。然而，将光疗和免疫疗法整合在用于癌症治疗的单一纳米剂中仍然是一项艰巨的任务。在这里，我们制作了（Cu ₉ S ₅ @mSiO ₂ -PpIX @ MnO ₂@CpG（CSPM @ CpG））作为光疗增强免疫疗法的协同治疗模型。细胞内胞嘧啶-磷酸-鸟嘌呤（CpG）的吸收促进了肿瘤组织中细胞毒性T淋巴细胞（CTL）的浸润，进一步刺激了干扰素γ（IFN-γ）的产生并显着提高了免疫应答水平。通过协同PTT / PDT /免疫疗法已获得了出色的抗肿瘤作用。CpG的免疫反应有效地抑制了肿瘤的转移。因此，我们提出的工作提供了一种将光疗与免疫疗法相结合的策略，以提高治疗效率并进一步抑制肿瘤的转移。