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DNA damage analysis concerning GSTM1 and GSTT1 gene polymorphism in gold jewellery workers from Peshawar Pakistan.
Biomarkers ( IF 2.6 ) Pub Date : 2020-07-12 , DOI: 10.1080/1354750x.2020.1791253
Muhammad Khisroon 1 , Ajmal Khan 1 , Asma Ayub 1 , Ihsan Ullah 2 , Javeed Farooqi 1 , Abid Ullah 1
Affiliation  

Purpose

To evaluate the genotoxic effects of gold jewellery fumes and its association with GSTM1 and GSTT1 genetic polymorphisms.

Materials and methods

We examined 94 subjects including 54 gold jewellery workers and 40 controls. The DNA damage was evaluated by alkaline comet assay and genotyping by PCR.

Results

The mean total comet score (TCS) in gold jewellery workers was significantly higher as compared to the control subjects (128.0 ± 60.6 versus 47.7 ± 21.4; p = 0.0001). Duration of occupational exposure had positive correlation (r = 0.453, p < 0.01) with DNA damage. Age and tobacco use had significant effects on the TCS of the exposed group as compared to the control group (p < 0.05). The frequency of the GSTM1-null genotype in the exposed group was significant (p = 0.004) as compared to the control group. No significant association (p > 0.05) between the GSTM1 and GSTT1 genotypes and DNA damage was found.

Conclusions

Our results suggest that there is increased DNA damage in gold jewellery workers due to their occupational surroundings. Hence there is a strong need to educate the workers about the adverse health effects of potentially hazardous chemicals and highlight the importance of using protective measures.



中文翻译:

来自巴基斯坦白沙瓦的金饰工人的GSTM1和GSTT1基因多态性的DNA损伤分析。

目的

评估黄金珠宝烟雾的遗传毒性作用及其与GSTM1和GSTT1遗传多态性的关联。

材料和方法

我们检查了94位受试者,其中包括54位金饰珠宝工人和40位对照。通过碱性彗星试验评估DNA损伤,并通过PCR进行基因分型。

结果

与对照组相比,金饰珠宝工作者的平均总彗星得分(TCS)显着更高(128.0±60.6与47.7±21.4;p  = 0.0001)。职业暴露的持续时间 与DNA损伤呈正相关(r  = 0.453,p <0.01)。与对照组相比,年龄和吸烟对暴露组的TCS有显着影响(p  <0.05)。 与对照组相比,暴露组中GSTM1-null基因型的频率显着(p = 0.004)。 在GSTM1和GSTT1基因型与DNA损伤之间未发现显着关联(p > 0.05)。

结论

我们的结果表明,由于金饰工人的职业环境,其DNA损伤增加。因此,非常需要对工人进行有关潜在危险化学品的不良健康影响的教育,并强调使用保护性措施的重要性。

更新日期:2020-08-08
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