To investigate the effects of oral bacteriotherapy on intestinal phenylalanine and tyrosine metabolism, in this longitudinal, double-arm trial, 15 virally suppressed HIV+ individuals underwent blood and fecal sample collection at baseline and after 6 months of oral bacteriotherapy. A baseline fecal sample was collected from 15 healthy individuals and served as control group for the baseline levels of fecal phenylalanine and tyrosine. CD4 and CD8 immune activation (CD38⁺) was evaluated by flow cytometry. Amino acid evaluation on fecal samples was conducted by Proton Nuclear Magnetic Resonance. Results showed that HIV+ participants displayed higher baseline phenylalanine/tyrosine ratio values than healthy volunteers. A significand reduction in phenylalanine/tyrosine ratio and peripheral CD4⁺ CD38⁺ activation was observed at the end of oral bacteriotherapy. In conclusion, probiotics beneficially affect the immune activation of HIV+ individuals. Therefore, the restoration of intestinal amino acid metabolism could represent the mechanisms through which probiotics exert these desirable effects.

中文翻译：

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HIV-1感染的神经认知障碍患者中苯丙氨酸和酪氨酸代谢的调节：来自临床试验的结果。

为了研究口服细菌治疗对肠道苯丙氨酸和酪氨酸代谢的影响，在这项纵向双臂试验中，有15个病毒抑制的HIV +个体在基线和口服细菌治疗6个月后接受了血液和粪便样本的收集。从15名健康个体中收集粪便基线样本，并将其作为粪便苯丙氨酸和酪氨酸基线水平的对照组。通过流式细胞术评估CD4和CD8的免疫激活（CD38 ⁺）。通过质子核磁共振对粪便样品进行氨基酸评估。结果表明，与健康志愿者相比，HIV +参与者显示出更高的基线苯丙氨酸/酪氨酸比值。苯丙氨酸/酪氨酸比率和外周CD4 ⁺显着降低口服细菌治疗结束时观察到CD38 ⁺激活。总之，益生菌有益地影响HIV +个体的免疫激活。因此，肠道氨基酸代谢的恢复可能代表了益生菌发挥这些理想作用的机制。