The purpose of this study was to set up a sensitive method for the simultaneous determination of phycotoxins and cyanotoxins—emerging pollutants with different structures and harmful properties (hepatotoxicity, neurotoxicity and cytotoxicity)—in environmental waters. Due to the low concentrations detected in these samples, a pre-concentration step is required and here it was performed in a single step with a commercial cartridge (Strata™-X), achieving enrichment factors up to 200 and satisfactory recovery (R = 70-118%) in different aqueous matrices. After solid-phase extraction (SPE), toxins were separated and quantified by High Performance Liquid Chromatography- Heated ElectroSpray Ionisation Tandem Mass Spectrometry (HPLC-HESI-MS/MS) in Multiple Reaction Monitoring (MRM) mode. An analytical evaluation of the proposed method was done based on the analytical figures of merit, such as precision and trueness, linearity, selectivity, and sensitivity, and it turned out to be a robust tool for the quantification of ng L^-1 levels, phycotoxins and cyanotoxins in both freshwater and saltwater samples.

中文翻译：

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内陆和沿海水域中藻毒素和氰毒素的同时预浓缩和HPLC-MS / MS定量分析。

这项研究的目的是建立一种灵敏的方法，用于同时测定环境水中的藻毒素和氰毒素（新兴的具有不同结构和有害特性（肝毒性，神经毒性和细胞毒性）的污染物）。由于在这些样品中检测到的低浓度，需要进行预浓缩步骤，此处使用商业化试剂盒（Strata™-X）在一个步骤中进行，可实现高达200的富集因子和令人满意的回收率（R = 70 -118％）在不同的水性基质中。固相萃取（SPE）后，通过多反应监测（MRM）模式下的高效液相色谱加热电喷雾串联质谱（HPLC-HESI-MS / MS）分离和定量毒素。淡水和盐水样品中的^-1含量，藻毒素和氰毒素。