Many Trichoderma spp. are successful plant beneficial microbial inoculants due to their ability to act as biocontrol agents with direct antagonistic activities to phytopathogens, and as biostimulants capable of promoting plant growth. This work investigated the effects of treatments with three selected Trichoderma strains (T22, TH1, and GV41) to strawberry plants on the productivity, metabolites and proteome of the formed fruits. Trichoderma applications stimulated plant growth, increased strawberry fruit yield, and favored selective accumulation of anthocyanins and other antioxidants in red ripened fruits. Proteomic analysis of fruits harvested from the plants previously treated with Trichoderma demonstrated that the microbial inoculants highly affected the representation of proteins associated with responses to stress/external stimuli, nutrient uptake, protein metabolism, carbon/energy metabolism and secondary metabolism, also providing a possible explanation to the presence of specific metabolites in fruits. Bioinformatic analysis of these differential proteins revealed a central network of interacting molecular species, providing a rationale to the concomitant modulation of different plant physiological processes following the microbial inoculation. These findings indicated that the application of Trichoderma-based products exerts a positive impact on strawberry, integrating well with previous observations on the molecular mechanisms activated in roots and leaves of other tested plant species, demonstrating that the efficacy of using a biological approach with beneficial microbes on the maturing plant is also able to transfer advantages to the developing fruits.

许多 木霉属spp。它们是成功的植物有益微生物接种剂，因为它们具有充当对植物病原体具有直接拮抗活性的生物防治剂的能力，以及能够促进植物生长的生物刺激剂。这项工作调查了三种选择的治疗效果木霉属 菌株（T22，TH1和GV41）对草莓果实形成的生产力，代谢产物和蛋白质组的影响。 木霉属施用刺激了植物的生长，增加了草莓果实的产量，并有利于花红素和其他抗氧化剂在红色成熟果实中的选择性积累。从先前用过的植物中收获的果实收获的蛋白质组学分析木霉属证明了微生物接种剂会极大地影响与应激/外部刺激，营养吸收，蛋白质代谢，碳/能量代谢和次级代谢相关的蛋白质的表达，也为水果中特定代谢物的存在提供了可能的解释。这些差异蛋白的生物信息学分析揭示了相互作用分子种类的中心网络，为微生物接种后伴随的不同植物生理过程的调节提供了理论依据。这些发现表明木霉属的产品对草莓产生了积极的影响，与先前对其他受试植物的根和叶中激活的分子机制的观察结果很好地结合在一起，证明了对成熟植物使用生物方法和有益微生物的功效也能够将优势转移到发展中的果实。