The Vietnamese Ban pig is a precious genetic resource that needs to be preserved. In vitro embryo production from in vitro matured (IVM) oocytes is an important tool for the utilization of cryopreserved porcine sperm. The aim of this study was to compare two media for the IVM of Ban pig oocytes. Immature oocytes were subjected to IVM either in a non‐defined (TCM‐199 + pig follicular fluid) or in a defined base medium (POM + epidermal growth factor). At the end of IVM, the oocytes were in vitro fertilized (IVF) with frozen Ban sperm. Ten hours after IVF, the oocytes were either subjected to orcein staining to check fertilization and maturation status or cultured in vitro for 7 days. There was no difference between the two IVM media in terms of percentages of oocyte maturation and blastocyst production. However, the percentage of male pronuclear formation after IVF and the total cell numbers in blastocysts were higher with the defined system. Zygotes obtained by the two IVM systems survived vitrification at similar rates. In conclusion, the two IVM systems were both effective for the production of Ban pig embryos; however, better embryo quality was achieved with the defined one.

中文翻译：

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越南本土板猪体外胚胎生产和受精卵玻璃化的优化：不同卵母细胞体外成熟系统的影响。

越南班猪是一种需要保存的珍贵遗传资源。体外成熟 (IVM) 卵母细胞的体外胚胎生产是利用冷冻保存的猪精子的重要工具。本研究的目的是比较两种培养基对 Ban 猪卵母细胞的 IVM。未成熟卵母细胞在非限定（TCM-199 + 猪卵泡液）或限定基础培养基（POM + 表皮生长因子）中进行 IVM。在 IVM 结束时，卵母细胞用冷冻的 Ban 精子进行体外受精 (IVF)。体外受精后 10 小时，卵母细胞要么接受地衣红染色以检查受精和成熟状态，要么在体外培养 7 天。两种 IVM 培养基在卵母细胞成熟百分比和胚泡生成百分比方面没有差异。然而，体外受精后雄性原核形成的百分比和囊胚中的总细胞数在定义的系统中更高。两种 IVM 系统获得的受精卵以相似的速度在玻璃化冷冻中存活下来。综上所述，两种IVM系统对Ban猪胚胎的生产均有效；然而，定义的胚胎质量更好。