Scar free healing together with pain management is one of the major considerations in full thickness wound healing. Extensive wounds take longer to heal without any clinical intervention and, hence, need natural or artificial extracellular matrix support for quick skin regeneration. To address these issues, medicated 3D porous biomimetic scaffolds were developed with a unique combination of biopolymers, that is, chitosan, sodium alginate, and elastin, supplemented with a non‐steroidal anti‐inflammatory drug (NSAID). Scaffolds were physically characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), swelling ratio analysis, and degradation studies. Findings of the performed analyses proved that these skin substitutes suitable for skin tissue engineering applications attributable to their nano‐microporous structures (pore size in range of 0.085–256 μm) allowing cell infiltration and high‐water absorption capacity for management of wound exudates. Optimal dose of the loaded ibuprofen was estimated by evaluating effect of variable concentrations of ibuprofen (control, ILM‐10, ILM‐15, and ILM‐20) on adipose tissue‐derived mesenchymal stem cells (ASCs) proliferation rate. Out of all experimental groups, ILM‐20 constructs were found to accelerate the proliferation rate of seeded ASCs confirming their non‐cytotoxic characteristics as well potential to be used for translational scaffold‐based therapies.

中文翻译：

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用于皮肤组织工程应用的载有 NSAID 的纳米复合支架的开发。

无疤痕愈合和疼痛管理是全层伤口愈合的主要考虑因素之一。在没有任何临床干预的情况下，大面积伤口需要更长的时间才能愈合，因此，需要天然或人工细胞外基质支持以实现快速皮肤再生。为了解决这些问题，开发了含药 3D 多孔仿生支架，采用独特的生物聚合物组合，即壳聚糖、海藻酸钠和弹性蛋白，并辅以非甾体抗炎药 (NSAID)。通过扫描电子显微镜 (SEM)、傅里叶变换红外光谱 (FTIR)、溶胀率分析和降解研究对支架进行物理表征。所进行的分析结果证明，这些皮肤替代品适用于皮肤组织工程应用，这归因于它们的纳米微孔结构（孔径在 0.085-256 μm 范围内）允许细胞浸润和用于管理伤口渗出液的高吸水能力。通过评估不同浓度的布洛芬（对照、ILM-10、ILM-15 和 ILM-20）对脂肪组织来源的间充质干细胞 (ASC) 增殖率的影响，估计负载的布洛芬的最佳剂量。在所有实验组中，发现 ILM-20 构建体可以加速接种的 ASC 的增殖速率，证实了它们的非细胞毒性特征以及用于基于转化支架的疗法的潜力。085–256 μm）允许细胞浸润和高吸水能力，用于处理伤口渗出液。通过评估不同浓度的布洛芬（对照、ILM-10、ILM-15 和 ILM-20）对脂肪组织来源的间充质干细胞 (ASC) 增殖率的影响，估计负载的布洛芬的最佳剂量。在所有实验组中，发现 ILM-20 构建体可以加速接种的 ASC 的增殖速率，证实了它们的非细胞毒性特征以及用于基于转化支架的疗法的潜力。085–256 μm）允许细胞浸润和高吸水能力，用于处理伤口渗出液。通过评估不同浓度的布洛芬（对照、ILM-10、ILM-15 和 ILM-20）对脂肪组织来源的间充质干细胞 (ASC) 增殖率的影响，估计负载的布洛芬的最佳剂量。在所有实验组中，发现 ILM-20 构建体可以加速接种的 ASC 的增殖速率，证实了它们的非细胞毒性特征以及用于基于转化支架的疗法的潜力。