Reoviruses (respiratory enteric orphan viruses) are nonenveloped viruses with segmented dsRNA genome. Viruses in the family Reoviridae are quite stable in the environment. Recently, they have been identified with various pathologies and physiologic dysfunctions in a wide range of organs and tissues, including the hepatobiliary system, the myocardium, lungs, and endocrine tissues. Although most cases of reovirus infection are mild or subclinical diseases, the prevention measures are currently needed, especially for young children suffering from dehydrating gastroenteritis. To inhibit viral replication, different RNases targeting viral RNA are proposed. Here, we first have shown that RNase from Bacillus pumilus (binase) acts as an antiviral agent at the level of the whole animal organism infected by Mammalian orthoreovirus 1 strain Lang (TL1). The results obtained on the mice model infected with 10 LD₅₀ and 20 LD₅₀ doses of reovirus indicate the restoration of mice physiological parameters under binase treatment at the dose of 50 μg/mouse. Thus, our research supports the relevance of binase as a promising antiviral agent that affects viral RNA.

呼肠孤病毒（呼吸道肠道孤儿病毒）是具有分段dsRNA基因组的非包膜病毒。呼肠孤病毒科中的病毒在环境中非常稳定。最近，已在广泛的器官和组织（包括肝胆系统，心肌，肺和内分泌组织）中发现它们具有各种病理学和生理功能障碍。尽管大多数呼肠孤病毒感染病例是轻度或亚临床疾病，但目前仍需要采取预防措施，特别是对于患有脱水性胃肠炎的幼儿。为了抑制病毒复制，提出了靶向病毒RNA的不同RNA酶。在这里，我们首先显示了来自短小芽孢杆菌的RNase（binase）作为抗病毒剂，在被哺乳动物正咽病毒1病毒Lang（TL1）感染的整个动物有机体中发挥作用。在用10 LD ₅₀和20 LD ₅₀剂量的呼肠孤病毒感染的小鼠模型上获得的结果表明，在以50μg/小鼠的剂量进行酶酶处理后，小鼠的生理参数得以恢复。因此，我们的研究支持binase作为影响病毒RNA的有前途的抗病毒药物的相关性。