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Identification, biochemical composition and phycobiliproteins production of Chroococcidiopsis sp. from arid environment
Process Biochemistry ( IF 4.4 ) Pub Date : 2020-10-01 , DOI: 10.1016/j.procbio.2020.07.005
Zaida Montero-Lobato , Juan L. Fuentes , Inés Garbayo , Carmen Ascaso , Jacek Wierzchos , José M. Vega , Carlos Vílchez

Abstract Molecular and microscopic studies were performed to identify Chroococcidiopsis sp., an endolithic cyanobacterium, isolated from gypsum rocks of Atacama Desert (Chile). It was adapted to grow in mineral liquid medium, with 9 mM nitrate, bubbled with CO2-enriched air (2.5 % v/v), and continuously illuminated with a white light of 70 μmol photons m–2 s–1. The obtained biomass (productivity of 0.21 g L–1 d–1) had a C/N ratio of 6.67, and it contained carbohydrates (45.40 % of dry weight), proteins (36.72 %), lipids (5.60 %) nucleic acids (3.90 %) and ashes (8.28 %). The lipid fraction was particularly rich in palmitic (29.86 % of total fatty acids), linoleic (18.20 %), palmitoleic (12.75 %), linolenic (10.92 %), stearic (9.64 %) and capric acid (6.29 %). Chroococcidiopsis sp. accumulated phycobiliproteins in a light-dependent process and produced 204 mg g–1, under incident light of 10 μmol photons·m–2·s–1, with a relative abundance of 40.9 % for phycocyanin, 23.3 % for phycoerythrin, and 35.8 % for allophycocyanin. The biomass from this cyanobacterium can be a good source of these pigments, especially APC (maximum of 95 mg g dw−1), which are of interest for pharmacological, cosmetic, and food industries.

中文翻译:

Chroococcidiopsis sp. 的鉴定、生化成分和藻胆蛋白的产生。来自干旱环境

摘要 进行了分子和显微研究以鉴定从阿塔卡马沙漠(智利)的石膏岩中分离的内石蓝细菌 Chroococcidiopsis sp.。它适合在含有 9 mM 硝酸盐、富含 CO2 的空气 (2.5 % v/v) 的矿物液体培养基中生长,并用 70 μmol 光子 m-2 s-1 的白光连续照射。获得的生物质(生产力为 0.21 g L–1 d–1)的 C/N 比为 6.67,它包含碳水化合物(干重的 45.40%)、蛋白质(36.72%)、脂质(5.60%)、核酸( 3.90 %) 和灰烬 (8.28 %)。脂质部分特别富含棕榈酸(占总脂肪酸的 29.86%)、亚油酸(18.20%)、棕榈油酸(12.75%)、亚麻酸(10.92%)、硬脂酸(9.64%)和癸酸(6.29%)。Chroococcidiopsis sp. 在光依赖过程中积累藻胆蛋白并在 10 μmol 光子·m-2·s-1 入射光下产生 204 mg g-1,藻蓝蛋白的相对丰度为 40.9%,藻红蛋白的相对丰度为 23.3%,和 35.8%为别藻蓝蛋白。来自这种蓝藻的生物质可以是这些色素的良好来源,尤其是 APC(最大 95 mg g dw-1),它们对药理学、化妆品和食品工业很感兴趣。
更新日期:2020-10-01
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