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An ATP binding cassette transporter HvABCB25 confers aluminum detoxification in wild barley.
Journal of Hazardous Materials ( IF 13.6 ) Pub Date : 2020-07-03 , DOI: 10.1016/j.jhazmat.2020.123371
Wenxing Liu 1 , Xue Feng 1 , Fangbin Cao 2 , Dezhi Wu 1 , Guoping Zhang 1 , Eva Vincze 3 , Yizhou Wang 1 , Zhong-Hua Chen 4 , Feibo Wu 2
Affiliation  

Aluminum (Al) stress in acid soils is one of the major factors limiting crop productivity. ATP binding cassette (ABC) transporters have numerous roles in plants, but the link between ABCB protein subfamily and plant Al tolerance is still elusive. Here, we identified and characterized a novel tonoplast HvABCB25 in barley root cells. HvABCB25 was up-regulated in the transcriptome of Al-tolerant wild barley XZ16 under Al treatment and was highly Al-inducible in root tips. ABCB25 is originated from Streptophyte algae and evolutionarily conserved in land plants. Moreover, silencing HvABCB25 in Al-tolerant XZ16 led to significant suppression of Al tolerance as indicated by significantly reduced root growth and enhanced Al accumulation in root cells. Conversely, HvABCB25-overexpressed plants and Golden Promise showed similar Al content in whole roots and in cell sap, but the overexpression lines exhibited significantly higher Al-induced relative root growth and dry weight. Al florescence in cytosol of root cells were significantly less in overexpression lines than that in GP. These results indicated that overexpressing HvABCB25 may be responsible for Al detoxification via vacuolar Al sequestration in barley roots, providing useful insight into the genetic basis for a new Al detoxification mechanism towards plant Al tolerance in acid soils.



中文翻译:

ATP结合盒转运蛋白HvABCB25赋予野生大麦铝解毒作用。

酸性土壤中的铝胁迫是限制作物生产力的主要因素之一。ATP结合盒(ABC)转运蛋白在植物中起着许多作用,但ABCB蛋白亚家族与植物A1耐性之间的联系仍然难以捉摸。在这里,我们在大麦根细胞中鉴定并鉴定了一种新型的液泡膜HvABCB25。HvABCB25上调在Al耐受型野生大麦XZ16下铝处理的转录和在根尖高Al诱导。ABCB25源自链球菌藻类,并在陆地植物中进化保存。此外,在耐铝的XZ16中沉默HvABCB25导致对铝耐性的显着抑制,这表现为根生长明显减少和根细胞中铝积累增加。相反,HvABCB25过度表达的植物和Golden Promise在整个根和细胞液中显示出相似的铝含量,但过表达系表现出明显更高的铝诱导的相对根生长和干重。与GP相比,过表达系中根细胞胞质中的Al花序明显减少。这些结果表明,过表达的HvABCB25可能通过大麦根中液泡状铝的螯合来实现铝的解毒作用,从而为针对酸性土壤中植物铝耐受性的新型铝解毒机制的遗传基础提供了有用的见识。

更新日期:2020-07-03
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