The main flavonoid components of Radix Tetrastigma (RTF) were extracted and characterized by high performance liquid chromatography. In vitro, RTF suppressed the viability of A549 cells, and inhibited the invasion and migration of A549 by damaging the lamellipodium and the structure of F-actin. In vivo, compared to the model group, RTF inhibited the growth of tumor volumes in nude mice, and decreased the weight of tumor. To uncover the inner mechanism, the proliferation-related proteins: PCNA, Ki67 and the apoptosis-related proteins: caspase-3, caspase-9, Bax and Bcl-2 were examined by immunohistochemical and western blot analysis. Compared to the model group, the production levels of PCNA, Ki67 and Bcl-2 were significantly down-regulated by RTF, and the expression levels of caspase-3, caspase-9 and Bax were markedly up-regulated. Comprehensively, RTF could suppress the proliferation of A549 and promote the apoptosis of A549, suggesting RTF as a potential resource to fight against non-small cell lung cancer (NSCLC).

的主要的类黄酮成分沙参崖（RTF）提取和特征是高效液相色谱法。在体外，RTF通过破坏层状脂质体和F-肌动蛋白的结构，抑制A549细胞的活力，并抑制A549的侵袭和迁移。体内与模型组相比，RTF抑制了裸鼠体内肿瘤体积的生长，并减轻了肿瘤的重量。为了揭示其内在机理，通过免疫组织化学和蛋白质印迹分析检查了增殖相关蛋白：PCNA，Ki67和凋亡相关蛋白：caspase-3，caspase-9，Bax和Bcl-2。与模型组相比，RTF显着下调了PCNA，Ki67和Bcl-2的生产水平，而caspase-3，caspase-9和Bax的表达水平显着上调。全面而言，RTF可以抑制A549的增殖并促进A549的凋亡，这表明RTF可作为对抗非小细胞肺癌（NSCLC）的潜在资源。