Edible insects have been important sources of food proteins for human consumption and animal feed. In this study, a protein isolate from Pterophylla beltrani Bolivar & Bolivar, 1942 (Orthoptera: Tettigoniidae) was enzymatically processed and its nutraceutical properties were evaluated. Protein isolates were obtained from an insect flour and then was hydrolyzed for 5 h with a sequential process using pepsin and trypsin-chymotrypsin to simulate the gastric intestinal fluids. To evaluate the effect of peptide molecular size on nutraceutical properties, the peptides obtained from Total hydrolyzed (TH) were fractionated by ultrafiltration (UF) with 10 kDa and 3 kDa UF membranes giving fractions (F) with different molecular size (F < 3KDa, F < 10KDa and F > 10KDa). The inhibition assay of Angiotensin-Converting Enzyme (ACE) showed that the best treatment (P < 0.05) was the (TH) with an IC₅₀ value of 0.5 mg/mL while the F < 3KDa was the lowest (P < 0.05) with an IC50 of 1.44 mg/mL, and the peptide size had no effect. However, an α-amylase inhibition was observed with an increase of the IC50 value between TH, F > 10KDa and F < 10KDa although no significative difference (P > 0.05) was found between the TH and F < 3KDa with IC50 of 0.48 and 0.68 mg/mL, respectively. In antioxidant activity, significant differences (P < 0.05) were observed between TH and UF fractions where the best response was in the F < 3KDa. In conclusion, P. beltrani proteins isolate are a source of bioactive peptide, and these could be considered as potential edible insect and sustainable food with nutraceutical effects.

食用昆虫已经成为人类食用和动物饲料的重要食物蛋白来源。在这项研究中，从蕨叶蕨中分离出一种蛋白质Bolivar＆Bolivar，1942年（直翅目：Tettigoniidae）经过酶处理，并评估了其营养保健性质。从昆虫粉中获得蛋白质分离物，然后使用胃蛋白酶和胰蛋白酶-胰凝乳蛋白酶通过顺序过程将其水解5小时，以模拟胃中的肠液。为了评估肽分子大小对营养保健品性能的影响，将总水解（TH）所得的肽用10 kDa和3 kDa UF膜通过超滤（UF）进行分馏，得到具有不同分子大小的级分（F）（F <3KDa， F <10KDa和F> 10KDa）。血管紧张素转换酶（ACE）的抑制试验表明，最佳治疗（P <0.05）是使用IC ₅₀的（TH）值为0.5 mg / mL，而F <3KDa最低（P <0.05），IC50为1.44 mg / mL，并且肽大小没有影响。然而，尽管在TH和F <3KDa之间没有发现显着性差异（P> 0.05），但IC50分别为0.48和0.68，但TH，F> 10KDa和F <10KDa之间的IC50值增加时，观察到α-淀粉酶抑制作用。分别为mg / mL。在抗氧化剂活性方面，在TH和UF组分之间观察到显着差异（P <0.05），其中最佳响应在F <3KDa。总之，P。Beltrani蛋白分离物是生物活性肽的来源，可以被认为是潜在的食用昆虫和具有保健功效的可持续食品。