The covalent immobilization of xylanase onto the trichlorotriazine-functionalized polyethylene glycol grafted magnetic nanoparticles was exploited to generate a stabilized xylanase with improved catalytic activity and stability. Several tools were deployed to monitor the synthesis and immobilization processes, the loading capacity of nanocarrier, and the structural/chemical characteristics of the nanobiocatalyst. The optimum immobilization yield of xylanase was 260 mg xylanase/g nanocarrier in 20 mM phosphate buffer, pH 6.5 at 25 °C. A forward shift in optimum pH (6.5 to 7.5) and temperature (60 to 70 °C) of xylanase was observed after immobilization and the performance of immobilized enzyme was improved at high temperatures and pH's as affirmed by enhancement of v_max (2.69 to 6.01 U/mL) and decreases of E_a (14.61 to 13.41 kJ/mol). An increase in K_m from 25.51 to 40.42 mg/mL was recorded after immobilization. The obtained results indicated augmented thermal stability of the immobilized xylanase. Notably, it showed good reusability as validated by retention of 50% of its initial activity after nine recycles in enrichment of the pineapple juice clarification after 120 min incubation at 50 °C, pH 4.5. The structural analysis revealed some partial changes in the α-helix and β-sheet content of the enzyme after several recycles.

利用木聚糖酶共价固定在三氯三嗪官能化的聚乙二醇接枝的磁性纳米颗粒上以产生具有改善的催化活性和稳定性的稳定的木聚糖酶。部署了几种工具来监测合成和固定化过程，纳米载体的负载能力以及纳米生物催化剂的结构/化学特性。木聚糖酶的最佳固定化产量是在25 mC，pH 6.5的20 mM磷酸盐缓冲液中的260 mg木聚糖酶/ g纳米载体。固定后，观察到木聚糖酶的最佳pH（6.5至7.5）和温度（60至70°C）向前移动，固定化酶的性能在高温和pH值下得到了改善，这可以通过v _max的增加得到确认。（2.69至6.01 U / mL）和E _a的降低（14.61至13.41 kJ / mol）。固定后，K _m从25.51增加到40.42 mg / mL。获得的结果表明固定的木聚糖酶的热稳定性增强。值得注意的是，它显示出良好的可重复使用性，通过在50℃，pH 4.5的条件下孵育120分钟后浓缩菠萝汁澄清9次循环后保留了其初始活性的50％，证实了其可重复使用性。结构分析表明，经过几次循环后，酶的α-螺旋和β-折叠含量出现了部分变化。