A 28-day feeding trial was conducted to investigate the effects of Yu-Ping-Feng polysaccharides (YPS) containing Astragalus polysaccharides (APS), Atractylodes macrocephala polysaccharides (AMP) and Saposhnikoviae polysaccharides (SPS) on the immune response, intestinal microbiota, disease resistance and growth performance of Litopenaeus vannamei. Seven hundred and twenty shrimp (3.04 ± 0.33 g) were fed the following diets: Control, YPS1 (0.13% APS + 0.0325% AMP + 0.0325% SPS), YPS2 (0.13% APS + 0.0325% AMP + 0.065% SPS) and YPS3 (0.13% APS + 0.0325% AMP+0.0975% SPS). After 14 and 28 days of feeding, the immune responses of hemocytes and intestine were measured. Intestinal microbiota and growth performance were measured after 28 days of feeding, after that, a 7-day challenge test against Vibrio harveyi was conducted. A significant (P < 0.05) increase of the total haemocyte count (THC), phagocytic activity, antibacterial activity and phenoloxidase (PO) activity was observed in shrimp fed YPS diets compared to the control. Also, dietary YPS supplementation particularly YPS3 group significantly increased the expressions of immune-related genes in the hemocytes and intestine. Regarding the intestinal microbiota, the microbial diversity and richness decreased and functional genes associated with short-chain fatty acids metabolism increased in YPS groups. After Vibrio harveyi challenge, the cumulative mortality in YPS groups was significantly lower than that of the control. Besides, dietary YPS had no significant effect on growth performance of shrimp (P > 0.05). The present results suggested that YPS could be considered as potential prebiotics for aquaculture farmed shrimp.

进行了为期28天的喂养试验，以研究含黄芪多糖（APS），白术多糖（Atractylodes macrocephala多糖）（AMP）和鼠伤寒沙门氏菌多糖（SPS）的玉屏风多糖（YPS）对免疫应答，肠道菌群，疾病的影响凡纳滨对虾的抗性和生长性能。720只虾（3.04±0.33 g）的饮食如下：对照组，YPS1（0.13％APS + 0.0325％AMP + 0.0325％SPS），YPS2（0.13％APS + 0.0325％AMP + 0.065％SPS）和YPS3 （0.13％APS + 0.0325％AMP + 0.0975％SPS）。喂食14天和28天后，测量血细胞和肠道的免疫反应。喂食28天后测量肠道菌群和生长性能，然后进行7天针对哈维弧菌的攻击试验。显着性（P 与对照相比，在喂虾的YPS饲料中观察到总血细胞计数（THC），吞噬活性，抗菌活性和酚氧化酶（PO）活性增加（<0.05）。而且，饮食中的YPS补充剂，特别是YPS3组，显着增加了血细胞和肠中免疫相关基因的表达。关于肠道菌群，YPS组的微生物多样性和丰富度下降，与短链脂肪酸代谢相关的功能基因增加。在哈维氏弧菌攻击后，YPS组的累积死亡率显着低于对照组。此外，日粮YPS对虾的生长性能没有显着影响（P > 0.05）。目前的结果表明，YPS可以被认为是水产养殖虾的潜在益生元。