Juvenile hybrid grouper, Epinephelus lanceolatus ♂ × Epinephelus fuscoguttatus ♀ (mean weight: 26.5 ± 2.8 g, mean length: 11.8 ± 1.3 cm) were exposed to different, sub-lethal levels of waterborne ammonia (0, 1, 2, 4, and 8 mg NH₄⁺/L) for 2 weeks. We assessed the hematological parameters, antioxidant enzymes, and stress responses of juvenile hybrid grouper after 1 week and after 2 weeks. Hematological parameters such as hemoglobin and hematocrit levels, were significantly decreased by ammonia exposure. Plasma components such as the magnesium and total protein contents, and the glutamic oxaloacetic transaminase and glutamic pyruvic transaminase activities were significantly altered by ammonia exposure; however, no changes in the magnesium levels were detected. Antioxidant responses, such as superoxide dismutase and glutathione S-transferase activities, were also significantly affected by ammonia exposure. Stress indicator levels, i.e., plasma cholesterol and heat shock protein 70 levels, were significantly increased by ammonia exposure. The results of this study indicated that ammonia exposure has toxic effects on juvenile hybrid grouper and affects their hematological parameters, antioxidant enzymes, and stress responses.

少年石斑鱼（Epinephelus lanceolatus♂ × Epinephelus fuscoguttatus♀）（平均体重：26.5±2.8 g，平均长度：11.8±1.3 cm）暴露于不同的致死水平的水性氨水（0、1、2、4和8毫克NH ₄ ⁺/ L）2周。我们评估了1周和2周后青少年杂交石斑鱼的血液学参数，抗氧化酶和应激反应。氨暴露会显着降低血液学参数，例如血红蛋白和血细胞比容水平。暴露于氨会显着改变血浆成分，例如镁和总蛋白质含量，以及谷氨酸草酰乙酸转氨酶和谷氨酸丙酮酸转氨酶的活性。但是，未检测到镁含量的变化。氨暴露也显着影响抗氧化剂的响应，例如超氧化物歧化酶和谷胱甘肽S-转移酶的活性。暴露于氨会显着增加应激指标的水平，即血浆胆固醇和热休克蛋白70的水平。