N-Deacetylated hyaluronan (daHA) has been widely investigated as a starting material to develop biomaterials with unique composition and performance. However, its structure elucidation remains a challenging task due to its polysaccharide nature. After the brief mention of its properties and preparation, this review critically evaluates different analytical methods and approaches to characterize this promising polysaccharide. A special attention is paid to the determination of the degree of deacetylation. Besides, the analysis of its molecular weight, primary structure, and deacetylation pattern is also described. The older procedures are compared with the advanced techniques to provide reliable description of daHA.

N-脱乙酰透明质酸（daHA）已被广泛研究为开发具有独特组成和性能的生物材料的起始材料。然而，由于其多糖性质，其结构解析仍然是一项艰巨的任务。在简要介绍了其特性和制备方法后，本综述严格评估了表征该有前途的多糖的不同分析方法和方法。要特别注意脱乙酰度的确定。此外，还描述了其分子量，一级结构和脱乙酰化模式的分析。将较旧的过程与先进的技术进行比较，以提供对daHA的可靠描述。