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Cholecystokinin and bombesin activate neuronatin neurons in the nucleus of the solitary tract.
Brain Research ( IF 2.9 ) Pub Date : 2020-07-03 , DOI: 10.1016/j.brainres.2020.147006
Malika Guggenberger 1 , Kim-Marie Engster 1 , Tobias Hofmann 1 , Matthias Rose 1 , Andreas Stengel 2 , Peter Kobelt 1
Affiliation  

Neuronatin (Nnat) is involved in the regulation of cellular molecular signaling and appears to be also linked to metabolic processes. The gastrointestinal peptides cholecystokinin (CCK) and bombesin (BN) have an effect on the short-term inhibition of food intake and induce neuronal activation in different brain nuclei, prominently in the nucleus of the solitary tract (NTS) involved in the modulation of food intake. The aim of the study was to examine if Nnat immunoreactivity is detectable in the NTS, and whether peripheral CCK-8S or BN cause c-Fos activation of Nnat neurons. Non-fasted male Sprague-Dawley rats received an intraperitoneal (i.p.) injection of 5.2 or 8.7 nmol CCK-8S/kg or 26 or 32 nmol BN/kg (n = 4 all groups) or vehicle solution (0.15 M NaCl; n = 7). The number of c-Fos neurons was determined 90 min post injection in the NTS and dorsal motor nucleus of the vagus (DMV). We observed Nnat immunoreactive neurons in the NTS and DMV. CCK-8S (25-fold and 51-fold, p = 0.025 and p = 0.001) and BN (31-fold and 59-fold, p = 0.007 and p = 0.001) at both doses increased the number of c-Fos positive neurons in the NTS. CCK and BN did not show a significant effect in the DMV. Both doses of CCK-8S (24-fold and 48-fold p = 0.011 and p = 0.001) and bombesin (31-fold and 56-fold, p = 0.002 and p = 0.001) increased the number of activated Nnat neurons in the NTS (p = 0.001) compared to the vehicle group, while in the DMV no significant increase of c-Fos activation was detected. In conclusion, i.p. injected CCK-8S or BN induce an increased neuronal activity in NTS Nnat neurons, giving rise that Nnat may play a role in the regulation of food intake mediated by peripheral CCK-8S or BN.



中文翻译:

胆囊收缩素和铃蟾肽激活孤束核中的神经元。

Neuronatin (Nnat) 参与细胞分子信号的调节,似乎也与代谢过程有关。胃肠肽缩胆囊素 (CCK) 和铃蟾肽 (BN) 对食物摄入的短期抑制有影响,并诱导不同脑核中的神经元激活,尤其是在参与食物调节的孤束核 (NTS) 中录取。该研究的目的是检查 NTS 中是否可检测到 Nnat 免疫反应性,以及外周 CCK-8S 或 BN 是否会导致 Nnat 神经元的 c-Fos 激活。未禁食的雄性 Sprague-Dawley 大鼠接受 5.2 或 8.7 nmol CCK-8S/kg 或 26 或 32 nmol BN/kg(n = 4 所有组)或载体溶液(0.15 M NaCl;n = 7)。在 NTS 和迷走神经背运动核 (DMV) 注射后 90 分钟确定 c-Fos 神经元的数量。我们在 NTS 和 DMV 中观察到 Nnat 免疫反应神经元。CCK-8S(25倍和51倍,p  = 0.025 和p  = 0.001)和 BN(31 倍和 59 倍,p  = 0.007 和p  = 0.001)在两种剂量下都增加了 NTS 中 c-Fos 阳性神经元的数量。CCK 和 BN 在 DMV 中没有显示出显着影响。两种剂量的 CCK-8S(24 倍和 48 倍p  = 0.011 和p  = 0.001)和铃蟾肽(31 倍和 56 倍,p  = 0.002 和p  = 0.001)都增加了激活的 Nnat 神经元数量NTS ( p = 0.001) 与载体组相比,而在 DMV 中没有检测到 c-Fos 激活的显着增加。总之,ip 注射 CCK-8S 或 BN 诱导 NTS Nnat 神经元的神经元活动增加,从而表明 Nnat 可能在外周 CCK-8S 或 BN 介导的食物摄入调节中发挥作用。

更新日期:2020-07-13
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