The study describes the effects of administration of Queso Blanco cheese containing Bifidobacterium longum on the fecal microbiota, metabolite and serum cytokine in healthy beagle dogs. Twelve healthy beagle dogs were randomly divided in three groups of four dogs each:a control group, not fed with any cheese, and groups fed with Queso Blanco cheese with B. longum KACC 91563 (QCB) or without B. longum (QC) for 8 weeks. Fecal microbiota was analyzed using a culture-based method and 16s rRNA gene sequencing. Serum cytokine levels, activation of natural killer cells, and proliferation of peripheral blood mononuclear cells were determined. SPME-GC-MS method was used to determine the concentrations of short chain fatty acids and indole in dog feces. Administration of QCB for 4 weeks significantly increased the Bifidobacterium. QCB supplementation for 8 weeks reduceds Enterobacteriaceae and Clostridium perfringens (p < 0.05). The abundance of Fusobacterium, Blautia and Collinesella in QCB group were reduced as compared with the control group. Serum TNF-α and IL-6 levels at 8 weeks significantly increased in QCB group as compared with QC group. There was no change in the concentrations of total short chain fatty acids by B. longum at 0 and 4 weeks. At week 8, the acetic acid, propionic acid and butyric acid of the QCB and QC groups were significantly decreased compared to the control group. In conclusion, our results demonstrate that administration of QCB had positive effects on fecal microbiota and immune response in beagle dogs. We suggest that Queso Blanco cheese containing B. longum KACC 91563 could be used as a functional food for companion animals.

该研究描述了在健康的比格犬中施用长双歧杆菌的Queso Blanco奶酪对粪便微生物群，代谢产物和血清细胞因子的影响。将十二只健康的比格犬随机分为三组，每组四只：对照组，不饲喂任何干酪，以及饲喂Queso Blanco干酪，长双歧杆菌KACC 91563（QCB）或不加长双歧杆菌的组。（QC）8周。使用基于培养的方法和16s rRNA基因测序分析粪便微生物群。测定血清细胞因子水平，自然杀伤细胞的活化以及外周血单核细胞的增殖。用SPME-GC-MS法测定狗粪中短链脂肪酸和吲哚的浓度。QCB的管理4周显着增加了双歧杆菌。QCB补充8周可减少肠杆菌科细菌和产气荚膜梭菌（p <0.05）。丰梭杆菌，Blautia和Collinesella与对照组相比，QCB组的血脂减少。与QC组相比，QCB组在8周时的血清TNF-α和IL-6水平显着增加。在0和4周，长双歧杆菌的总短链脂肪酸浓度没有变化。在第8周，与对照组相比，QCB和QC组的乙酸，丙酸和丁酸显着降低。总之，我们的结果表明，QCB的给药对比格犬的粪便微生物群和免疫反应具有积极作用。我们建议包含长双歧杆菌KACC 91563的Queso Blanco奶酪可以用作陪伴动物的功能性食品。