In this study, 22 different fungal isolates associated with naturally dead insects of order Hemiptera including Bemisia tabaci, Aphis gossypii and Phenacoccus solenopsis were isolated from three different agroecological zones namely hot arid, cotton and central mixed zone. Fungal species were characterized morphologically on the basis of colony color, shape and spore structures through using different dichotomous keys. As a result fungi belonging to different species of Aspergillus, Acremonium, Beauveria, Cladosporium, Alternaria, Clonostachys, Penicillium, Trichoderma, Fusarium, Metarhizium and Verticillium were identified. All isolates of individual species were identical with respect to morphological differentiation. Principal component analysis (PCA) indicates strong correlation between Verticillium lecanii and Aspergillus fumigatus with respect to their isolation source (insect host). However, based on PCA, an uneven distribution of entomopathogenic fungi in different insect hosts is evident. Phylogenetic analysis revealed that Aspergillus fumigatus and A. nidulans are more closely related to different species of Penicillium, while Aspergillus oryzae and A. flavus formed separate clades, whereas different species of Alternaria, Cladosporium and Fusarium formed separate clades. Thus, the information generated will add to our understanding of the insect associated fungal communities for future development of potential biocontrol agents and their application as a vital component of sustainable agriculture.

在这项研究中，从三个不同的农业生态区（热干旱地区，棉花区和中部混合区）中分离了与半翅目自然死亡昆虫相关的22种不同真菌分离物，包括烟粉虱，棉蚜和斑节对虾。通过使用不同的二分键，根据菌落的颜色，形状和孢子结构在形态上表征真菌。作为属于不同物种的结果真菌曲霉属，支顶孢属，白僵菌，枝孢属，链格孢，Clonostachys，青霉属，木霉属，镰孢属，绿僵和黄萎病鉴定。就形态分化而言，单个物种的所有分离物都是相同的。主成分分析（PCA）表明，黄萎病菌和烟曲霉在其分离源（昆虫宿主）之间有很强的相关性。但是，基于PCA，昆虫病原真菌在不同昆虫宿主中的分布不均匀是很明显的。系统发育分析表明，烟曲霉和构巢曲霉更密切相关，不同物种的青霉，而米曲霉和黄曲霉形成独立的分支，而不同种类的黑斑，霉和镰刀菌形成单独的进化枝。因此，所产生的信息将增加我们对与昆虫相关的真菌群落的了解，以用于潜在的生物防治剂的未来开发及其作为可持续农业的重要组成部分的应用。