Sulforaphane (SFN) is a kind of natural isothiocyanate, which exists in cruciferous plants. Only few studies were about the anti-inflammatory effects of sulforaphane in ulcerative colitis. In this study, our purpose is to explore the effects of sulforaphane on the intestinal microbial community of UC mice. The severity of mice colitis were measured by colon length, survial rate, body weight and disease activity index (DAI) score. Histological and morphological evaluation of colon tissues were performed by HE. 16S rRNA gene amplicon pyrosequencing was used to analyza the changes of mouse flora. The variety of flora expression were explored using quantitative PCR. Sulforaphane treated mice had larger body weight and longer colon length than DSS-induced mice. The colon tissues of DSS group showed congestion and edema. Meanwhile, treatment with sulforaphane effectively reducted the damage scores and MPO activity. Sulforaphane reversed DSS-induced gut dysbiosis. Sulforaphane would shift the balance to Butyricicoccus on inflammation. The possible anti-inflammatory mechanism of sulforaphane is to coordinate with the probiotics such as Butyricicoccus. In summary, these findings proved that sulforaphane might be a useful content and serve as a potential therapy in the treatment of UC.

中文翻译：

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萝卜硫烷可改变微生物群并减轻DSS诱发的小鼠溃疡性结肠炎的结肠炎严重程度。

萝卜硫素（SFN）是一种天然的异硫氰酸盐，存在于十字花科植物中。关于萝卜硫烷在溃疡性结肠炎中的抗炎作用只有很少的研究。在这项研究中，我们的目的是探讨萝卜硫素对UC小鼠肠道微生物群落的影响。通过结肠长度，存活率，体重和疾病活动指数（DAI）评分来测量小鼠结肠炎的严重性。通过HE进行结肠组织的组织学和形态学评估。16S rRNA基因扩增子焦磷酸测序用于分析小鼠菌群的变化。使用定量PCR探索菌群表达的多样性。萝卜硫素治疗的小鼠比DSS诱导的小鼠具有更大的体重和更长的结肠长度。DSS组结肠组织充血水肿。与此同时，萝卜硫烷治疗有效降低了损伤评分和MPO活性。萝卜硫素逆转了DSS诱导的肠道营养不良。萝卜硫素会将余额转移到丁球菌对炎症。萝卜硫烷的可能的抗炎机制是与诸如Butyricicoccus的益生菌协调作用。总之，这些发现证明了萝卜硫烷可能是有用的内容，并且可以作为治疗UC的潜在疗法。