Non-thermal plasma (NTP) has a selective killing effect on a variety of cancer cells in vivo and in vitro , and is considered a potential tumor therapy. In this study, we found that metformin, which has shown promising antitumor effects in previous studies, with NTP synergistically increased cell death including apoptosis in colon cancer cell lines via reducing mitochondrial oxidative phosphorylation and intracellular adenosine triphosphate exhausting cellular energy, mediated through activation of AMP-activated protein kinase (AMPK). Reactive oxygen species was revealed to modulate the activation of AMPK after NTP exposure. Considering the safety and wide clinical use of metformin, the combination of metformin and NTP showed great potential in cancer therapy.

中文翻译：

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非热血浆与二甲双胍协同上调AMPK抑制能量代谢，从而诱导细胞死亡

非热血浆（NTP）在体内和体外对多种癌细胞具有选择性杀伤作用，被认为是一种潜在的肿瘤治疗方法。在这项研究中，我们发现二甲双胍在先前的研究中显示出有希望的抗肿瘤作用，与NTP协同增加了细胞死亡，包括结肠癌细胞系中的细胞凋亡，这是通过减少线粒体氧化磷酸化和胞内三磷酸腺苷耗尽细胞能量（通过激活AMP介导的）激活的蛋白激酶（AMPK）。在NTP暴露后，发现活性氧可调节AMPK的活化。考虑到二甲双胍的安全性和广泛的临床应用，二甲双胍和NTP的组合在癌症治疗中显示出巨大的潜力。