High proportion of human embryos produced by in vitro fertilization (IVF) is aneuploidy. Many factors are related to the prevalence of embryonic aneuploidies, such as maternal age, sperm quality, and in vitro manipulation of oocytes. Oocytes are usually inseminated by intracytoplasmic sperm injection (ICSI) procedures for preimplantation genetic testing. There is still no available information whether insemination procedures, regular IVF or ICSI, affect embryonic aneuploidies. In this case report, a patient at her age of 47 years old received donated oocytes from a young donor for infertility treatment. Half of oocytes were inseminated by regular IVF and other half of oocytes were inseminated by ICSI. Fertilized oocytes were cultured to blastocyst stage and then biopsied for preimplantation genetic testing for aneuploidies (PGT-A). The proportions of aneuploidies were compared between two insemination procedures. Forty-seven oocytes were retrieved, 23 were inseminated by regular IVF and 24 were removed from enclosed cumulus cells for ICSI. Out of 24 oocytes, 21 oocytes at metaphase II were inseminated by ICSI. After fertilization assessment, it was found that 12 oocytes from regular IVF fertilized normally. Nine blastocysts (75%) were biopsied and 1 (11.1%) was aneuploidy. By contrast, 19 out of 21 oocytes inseminated by ICSI fertilized normally, 14 blastocysts (73.7%) were obtained and 7 (50.0%) were aneuploidy. Transfer of a euploid blastocyst from regular IVF resulted in a healthy baby delivery. These results indicate that more embryos produced by ICSI are aneuploidy as compared with embryos produced by regular IVF. The results indicate that in vitro manipulation of oocytes for ICSI procedure may have adverse effect on human oocytes, and it may be one of the reasons causing aneuploid embryos in human IVF.

中文翻译：

---

体外受精的ICSI会导致更多的非整倍体胚胎吗？

体外受精（IVF）产生的高比例人类胚胎是非整倍性的。许多因素与胚胎非整倍体的流行有关，例如母体年龄、精子质量和卵母细胞的体外操作。卵母细胞通常通过胞浆内精子注射 (ICSI) 程序进行受精，以进行植入前基因检测。仍然没有可用的信息，授精程序、常规 IVF 或 ICSI 是否会影响胚胎非整倍体。在本病例报告中，一名 47 岁的患者接受了一位年轻供体捐赠的卵母细胞，用于治疗不孕症。一半的卵母细胞通过常规IVF受精，另一半卵母细胞通过ICSI受精。将受精的卵母细胞培养至囊胚阶段，然后进行活检以进行非整倍体的植入前基因检测 (PGT-A)。比较了两种授精程序之间的非整倍体比例。取回了 47 个卵母细胞，23 个通过常规 IVF 授精，24 个从封闭的卵丘细胞中取出用于 ICSI。在 24 个卵母细胞中，21 个处于中期 II 的卵母细胞通过 ICSI 受精。受精评估后发现，来自常规IVF的12个卵母细胞正常受精。9 个囊胚 (75%) 进行了活检，1 个 (11.1%) 是非整倍体。相比之下，通过ICSI受精的21个卵母细胞中有19个正常受精，获得了14个囊胚（73.7%），7个（50.0%）为非整倍体。从常规体外受精转移整倍体囊胚导致健康的婴儿分娩。这些结果表明，与常规 IVF 产生的胚胎相比，ICSI 产生的胚胎更多是非整倍体。