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An overlooked effect induced by surface modification: different molecular response of Chlorella pyrenoidosa to graphitized and oxidized nanodiamonds
Environmental Science: Nano ( IF 7.3 ) Pub Date : 2020-07-02 , DOI: 10.1039/d0en00444h
Chaofan Zhang 1, 2, 3, 4, 5 , Xiaochen Huang 2, 5, 6, 7, 8 , Yuhao Chu 1, 2, 3, 4, 5 , Nanqi Ren 1, 2, 3, 4, 5 , Shih-Hsin Ho 1, 2, 3, 4, 5
Affiliation  

The growing applications of carbon nanomaterials (CNMs) are speculated to exert potential risks to aquatic ecosystems. However, little is known about the response of aquatic microalgae to surface modified nanodiamonds (NDs), which could limit the safer design and scientific assessment for further application. Here, we comprehensively integrated phenotypic and transcriptional analyses to investigate the response of Chlorella pyrenoidosa to both graphitized nanodiamonds (GNDs) and oxidized nanodiamonds (ONDs). Results indicated that 50 mg L−1 GNDs could induce higher growth inhibition with severe organelle damage and oxidative stress than the OND treatment. Intriguingly, extracellular polymeric substances (EPSs) stimulated by OND exposure alleviated the adverse effects. Meanwhile, the conventional global molecular response to surface modified NDs was meticulously disclosed to fill in the knowledge gap. Additionally, the photosynthetic mechanism, amino acid synthesis, protein synthesis and protein export were further investigated under OND treatment, confirming a positive correlation for the crucial strategy to mitigate the negative effect. The underlying mechanism response to GNDs/ONDs has been thoroughly and completely revealed, providing a novel insight for the estimation of ND surface modification and the apperception of aquatic environmental risk.

中文翻译:

表面修饰引起的被忽略的效应:小球藻对石墨化和氧化的纳米金刚石的不同分子响应

碳纳米材料(CNMs)的不断增长的应用被认为会对水生生态系统造成潜在风险。然而,关于水生微藻类对表面改性纳米金刚石(NDs)的反应知之甚少,这可能会限制更安全的设计和科学评估以供进一步应用。在这里,我们全面整合了表型和转录分析,以研究小球藻对石墨化纳米金刚石(GNDs)和氧化纳米金刚石(ONDs)的响应。结果表明50 mg L -1与OND处理相比,GNDs可以诱导更高的生长抑制作用,并具有严重的细胞器损伤和氧化应激。有趣的是,OND暴露刺激了细胞外聚合物质(EPS)减轻了不利影响。同时,精心地公开了对表面修饰的NDs的常规全局分子响应,以填补知识空白。此外,在OND处理下,进一步研究了光合作用机制,氨基酸合成,蛋白质合成和蛋白质输出,从而确认了减轻不良影响的关键策略具有正相关性。对GNDs / ONDs的潜在机理反应已被彻底彻底地揭示,这为ND表面改性的估计和对水生环境风险的认识提供了新颖的见解。
更新日期:2020-08-14
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